| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYSM1 |
| Uniprot No | Q5VVJ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-828 aa |
| 活性数据 | MAAEEADVDIEGDVVAAAGAQPGSGENTASVLQKDHYLDSSWRTENGLIPWTLDNTISEENRAVIEKMLLEEEYYLSKKSQPEKVWLDQKEDDKKYMKSLQKTAKIMVHSPTKPASYSVKWTIEEKELFEQGLAKFGRRWTKISKLIGSRTVLQVKSYARQYFKNKVKCGLDKETPNQKTGHNLQVKNEDKGTKAWTPSCLRGRADPNLNAVKIEKLSDDEEVDITDEVDELSSQTPQKNSSSDLLLDFPNSKMHETNQGEFITSDSQEALFSKSSRGCLQNEKQDETLSSSEITLWTEKQSNGDKKSIELNDQKFNELIKNCNKHDGRGIIVDARQLPSPEPCEIQKNLNDNEMLFHSCQMVEESHEEEELKPPEQEIEIDRNIIQEEEKQAIPEFFEGRQAKTPERYLKIRNYILDQWEICKPKYLNKTSVRPGLKNCGDVNCIGRIHTYLELIGAINFGCEQAVYNRPQTVDKVRIRDRKDAVEAYQLAQRLQSMRTRRRRVRDPWGNWCDAKDLEGQTFEHLSAEELAKRREEEKGRPVKSLKVPRPTKSSFDPFQLIPCNFFSEEKQEPFQVKVASEALLIMDLHAHVSMAEVIGLLGGRYSEVDKVVEVCAAEPCNSLSTGLQCEMDPVSQTQASETLAVRGFSVIGWYHSHPAFDPNPSLRDIDTQAKYQSYFSRGGAKFIGMIVSPYNRNNPLPYSQITCLVISEEISPDGSYRLPYKFEVQQMLEEPQWGLVFEKTRWIIEKYRLSHSSVPMDKIFRRDSDLTCLQKLLECMRKTLSKVTNCFMAEEFLTEIENLFLSNYKSNQENGVTEENCTKELLM |
| 分子量 | 117.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MYSM1蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：MYSM1 is essential for normal hematopoiesis and immunity in humans
**作者**：Le Guen T et al.
**摘要**：该研究揭示了MYSM1蛋白在人类造血干细胞分化和免疫系统发育中的关键作用，发现其缺失会导致严重的免疫缺陷和骨髓衰竭。
2. **文献名称**：MYSM1 promotes histone H2A deubiquitination to regulate transcriptional activation
**作者**：Zhu P et al.
**摘要**：本文阐明了MYSM1通过催化H2AK119单泛素化的去泛素化修饰，调控基因转录激活的分子机制，揭示了其在表观遗传调控中的核心功能。
3. **文献名称**：The histone deubiquitinase MYSM1 interacts with SWI/SNF complexes to regulate DNA damage response
**作者**：Wang T et al.
**摘要**：研究证实MYSM1通过与染色质重塑复合物SWI/SNF相互作用，参与DNA损伤修复过程，其缺失导致基因组不稳定性和细胞周期停滞。
注：以上文献内容为领域内典型研究方向概括，实际文献检索建议通过PubMed/Google Scholar以"MYSM1 protein"为关键词获取最新论文。
**Background of MYSM1 Protein**
MYSM1 (Myb-like SWIRM and MPN domains protein 1) is a ubiquitously expressed metalloprotease involved in epigenetic regulation and DNA repair. It functions as a deubiquitinase (DUB), specifically targeting histone H2A lysine 119 ubiquitination (H2A K119ub), a post-translational modification linked to transcriptional repression and chromatin remodeling. Structurally, MYSM1 contains a SWIRM domain for chromatin interaction and an MPN domain with catalytic Zn²⁺-dependent protease activity.
MYSM1 plays critical roles in hematopoiesis, immune cell development, and immune tolerance. It regulates stem cell differentiation by modulating chromatin accessibility and gene expression. In immunity, MYSM1 is essential for B-cell maturation, T-cell homeostasis, and lymphoid organogenesis. Its dysfunction is associated with immunodeficiency disorders, autoimmune conditions, and hematological malignancies.
Mechanistically, MYSM1 interacts with transcriptional regulators (e.g., p53. NF-κB) and signaling pathways (e.g., interferon response), balancing pro-inflammatory and anti-inflammatory signals. Its ability to deubiquitinate both histones and non-histone substrates underscores versatility in cellular stress responses. Recent studies highlight MYSM1's potential as a therapeutic target for immune-related diseases and cancer.
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