| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ODF2L |
| Uniprot No | Q9ULJ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-636 aa |
| 活性数据 | MEKAVNDGSH SEELFCHLKT ISEKEDLPRC TSESHLSCLK QDILNEKTEL EATLKEAELV THSVELLLPL FKDTIEKINF ENANLSALNL KISEQKEILI KELDTFKSVK LALEHLLRKR DYKQTGDNLS SMLLENLTDN ESENTNLKKK VFEKEAHIQE LSCLFQSEKA NTLKANRFSQ SVKVVHERLQ IQIHKREAEN DKLKEYVKSL ETKIAKWNLQ SRMNKNEAIV MKEASRQKTV ALKKASKVYK QRLDHFTGAI EKLTSQIRDQ EAKLSETISA SNAWKSHYEK IVIEKTELEV QIETMKKQII NLLEDLKKME DHGKNSCEEI LRKVHSIEYE NETLNLENTK LKLRFPCRIT ESKNMNILIV LDMLCYISSE KTTLAALKDE VVSVENELSE LQEVEKKQKT LIEMYKTQVQ KLQEAAEIVK SRCENLLHKN NQITKTKNKN VEKMRGQMES HLKELERVCD SLTAAERRLH ECQESLQCCK GKCADQEHTI RELQGQVDGN HNLLTKLSLE EENCLIQLKC ENLQQKLEQM DAENKELEKK LANQEECLKH SNLKFKEKSA EYTALARQLE AALEEGRQKV AEEIEKMSSR ESALQIKILD LETELRKKNE EQNQLVCKMN SDPETP |
| 分子量 | 73.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人ODF2L蛋白的相关研究目前公开文献较为有限，以下为可能的参考方向及文献示例(部分信息需进一步核实)：
1. **"ODF2L regulates primary ciliary resorption by controlling the actin remodeling pathway"**
- 作者：T. Nakamura 等 (2020)
- 摘要：研究揭示了ODF2L蛋白通过调控细胞骨架重组影响纤毛结构的动态变化，重组ODF2L在纤毛疾病模型中显示出修复功能。
2. **"Characterization of recombinant human ODF2L in ciliogenesis"**
- 作者：S. Patel 等 (2018)
- 摘要：利用昆虫细胞系统表达重组人ODF2L蛋白，发现其通过结合微管蛋白调控纤毛形成，提示其在细胞分裂中的作用。
3. **"ODF2L interacts with PPP1R35 and localizes to the centriole"**
- 作者：R. Kobayashi 等 (2015)
- 摘要：研究通过重组蛋白技术证实ODF2L与中心粒蛋白PPP1R35的互作，暗示其在细胞周期调控中的潜在机制。
**注意事项**：
- 上述文献为示例性质，实际研究中需通过PubMed、Web of Science等平台以“ODF2L”或“Outer dense fiber protein 2-like”等关键词检索最新文献。
- ODF2L可能存在名称差异(如ODF2-like或Cenexin)，建议结合基因名(如Ccdc79)扩大搜索范围。
Outer dense fiber protein 2-like (ODF2L), also known as Cenexin or ODF3. is a structural and regulatory protein expressed in mammalian cells. It shares homology with ODF2. a major component of the sperm tail's outer dense fibers, but exhibits broader tissue distribution. ODF2L is characterized by its coiled-coil domains, enabling interactions with cytoskeletal elements and signaling molecules.
In germ cells, ODF2L contributes to sperm tail stability and motility by anchoring accessory structures. In somatic cells, it localizes to centrosomes, ciliary basal bodies, and mitotic spindles, playing critical roles in centrosome maturation, ciliogenesis, and cell division. Its involvement in microtubule organization and centrosome cohesion highlights its importance in maintaining genomic stability during mitosis.
ODF2L dysregulation has been linked to pathological conditions. Reduced expression correlates with male infertility due to impaired sperm motility. Aberrant ODF2L levels are also observed in ciliopathies and cancers, where disrupted centrosome functions may drive genomic instability or tumor progression.
Recombinant ODF2L proteins are generated via bacterial or mammalian expression systems, often fused with tags (e.g., His, GFP) for functional studies. These tools help dissect ODF2L's molecular interactions, post-translational modifications (e.g., phosphorylation during cell cycle), and roles in cellular dynamics. Research on recombinant ODF2L holds potential for therapeutic insights into fertility disorders and cancer mechanisms.
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