| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR52B2 |
| Uniprot No | Q96RD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-323 aa |
| 活性数据 | MSHTNVTIFHPAVFVLPGIPGLEAYHIWLSIPLCLIYITAVLGNSILIVVIVMERNLHVP MYFFLSMLAVMDILLSTTTVPKALAIFWLQAHNIAFDACVTQGFFVHMMFVGESAILLAM AFDRFVAICAPLRYTTVLTWPVVGRIALAVITRSFCIIFPVIFLLKRLPFCLTNIVPHSY CEHIGVARLACADITVNIWYGFSVPIVMVILDVILIAVSYSLILRAVFRLPSQDARHKAL STCGSHLCVILMFYVPSFFTLLTHHFGRNIPQHVHILLANLYVAVPPMLNPIVYGVKTKQ IREGVAHRFFDIKTWCCTSPLGS |
| 分子量 | 36.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR52B2蛋白的3-4篇文献示例(虚构内容，仅供格式参考)：
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1. **"Functional expression and ligand identification of human olfactory receptor OR52B2 in heterologous cells"**
*Zhang L, et al. (2017) Journal of Biological Chemistry*
摘要：报道了OR52B2在HEK293细胞中的重组表达策略，通过引入伴侣蛋白RTP1实现稳定膜定位，并筛选出该受体特异性响应中链脂肪酸(如癸酸)，证实其嗅觉信号通路激活能力。
2. **"Structural insights into the activation mechanism of OR52B2 via cryo-EM analysis"**
*De Marchi C, et al. (2020) Nature Communications*
摘要：利用冷冻电镜解析了OR52B2与其配体复合物的三维结构，揭示了该嗅觉受体结合脂肪酸后的构象变化及G蛋白偶联机制，为靶向设计调控分子提供依据。
3. **"Non-olfactory roles of OR52B2 in renal physiology"**
*Pluznick JL, et al. (2013) Proceedings of the National Academy of Sciences*
摘要：发现OR52B2在人类肾脏组织中表达，并通过体外重组实验证明其感知局部代谢物(如辛酸)，调控肾小球滤过功能，拓展了嗅觉受体在非嗅觉系统中的生理功能认知。
4. **"High-throughput deorphanization of human olfactory receptors using synthetic odor libraries"**
*Mainland JD, et al. (2015) Nature Neuroscience*
摘要：通过大规模配体筛选平台鉴定OR52B2为对中链挥发性脂肪酸(如己酸)高度敏感的受体，揭示其在食物气味感知中的潜在作用。
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注：上述内容为模拟文献，实际研究中需核实具体作者及期刊数据。如需真实文献，建议通过PubMed或Google Scholar检索关键词“OR52B2”、“human olfactory receptor 52B2”。
OR52B2 is a human olfactory receptor belonging to the G protein-coupled receptor (GPCR) superfamily. Encoded by the OR52B2 gene, it is part of the olfactory receptor subfamily 52. which is involved in detecting and transducing odorant signals in the nasal epithelium. As a chemosensory receptor, OR52B2 is thought to recognize specific volatile molecules, triggering intracellular signaling cascades that translate chemical stimuli into neural signals for odor perception. However, its precise ligand specificity and physiological role remain poorly characterized compared to other well-studied olfactory receptors.
Recombinant OR52B2 protein is artificially expressed in heterologous systems (e.g., bacterial, insect, or mammalian cells) for structural and functional studies. Its recombinant production enables research into receptor-ligand interactions, activation mechanisms, and potential extra-olfactory roles, as some olfactory receptors are implicated in non-sensory tissues. Challenges in studying OR52B2 include difficulties in achieving proper membrane localization and maintaining structural integrity during purification, common to many GPCRs. Current investigations focus on deorphaning the receptor (identifying its natural ligands) and exploring its possible applications in biosensors or targeted therapies. Despite limited understanding of its biological context, OR52B2 serves as a model for deciphering the molecular complexity of human olfaction and GPCR functionality.
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