| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM38 |
| Uniprot No | Q9H0Z9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-239aa |
| 氨基酸序列 | MLLQPAPCAP SAGFPRPLAA PGAMHGSQKD TTFTKIFVGG LPYHTTDASL RKYFEGFGDI EEAVVITDRQ TGKSRGYGFV TMADRAAAER ACKDPNPIID GRKANVNLAY LGAKPRSLQT GFAIGVQQLH PTLIQRTYGL TPHYIYPPAI VQPSVVIPAA PVPSLSSPYI EYTPASPAYA QYPPATYDQY PYAASPATAA SFVGYSYPAA VPQALSAAAP AGTTFVQYQA PQLQPDRMQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBM38重组蛋白的模拟参考文献示例(建议通过PubMed等数据库核实真实文献)：
1. **文献名称**：*RBM38 modulates p53 mRNA stability and limits colorectal cancer progression*
**作者**：Zhang Y, et al.
**摘要**：研究揭示RBM38通过直接结合p53 mRNA的3'UTR区域，增强其稳定性并促进p53蛋白表达，从而抑制结肠癌细胞增殖和转移。
2. **文献名称**：*RBM38 regulates cell cycle progression via post-transcriptional control of cyclins in hepatocellular carcinoma*
**作者**：Wang L, et al.
**摘要**：发现RBM38重组蛋白通过调控细胞周期相关基因(如Cyclin D1和p21)的mRNA稳定性，影响肝癌细胞的G1/S期转换，抑制肿瘤生长。
3. **文献名称**：*RBM38 interacts with PTEN mRNA to enhance its stability and suppress Akt signaling in breast cancer*
**作者**：Chen X, et al.
**摘要**：证明RBM38通过结合PTEN mRNA阻止其降解，上调PTEN蛋白水平，抑制乳腺癌中Akt信号通路活性，增强化疗敏感性。
4. **文献名称**：*RBM38 deficiency promotes DNA damage-induced apoptosis via destabilizing MDM2 mRNA*
**作者**：Liu H, et al.
**摘要**：阐明RBM38通过维持MDM2 mRNA稳定性调控p53-MDM2反馈环路，其缺失导致DNA损伤后细胞凋亡增加，提示其在基因组稳定性中的关键作用。
**注意**：以上文献为模拟概括，实际引用请以真实发表的论文为准。建议通过关键词“RBM38”“recombinant protein”“RNA-binding protein”在学术数据库中检索最新研究。
RBM38 (RNA-binding motif protein 38), also known as RNPC1. is a member of the RNA-binding protein family characterized by a conserved RNA recognition motif (RRM) domain. It plays a critical role in post-transcriptional gene regulation by interacting with target mRNAs to modulate their stability, splicing, and translation. Initially identified for its involvement in cell cycle control and apoptosis, RBM38 has been extensively studied in the context of cancer biology. It acts as a tumor suppressor or promoter depending on cellular context, primarily by regulating key oncogenes and tumor suppressors such as p53. c-Myc, and p21. For instance, RBM38 stabilizes p53 mRNA under stress conditions, enhancing p53-mediated tumor suppression, while its interaction with c-Myc mRNA can either promote or inhibit cancer progression in different tissue types.
Recombinant RBM38 protein is engineered for in vitro studies to elucidate its molecular mechanisms. Produced typically in Escherichia coli or mammalian expression systems, the purified protein retains RNA-binding activity and is used to investigate interactions with nucleic acids or partner proteins. Its applications span functional assays (e.g., electrophoretic mobility shift assays), structural studies, and high-throughput screening for therapeutic agents targeting RBM38-related pathways. Dysregulation of RBM38 has been linked to various cancers, including breast, colorectal, and lung malignancies, making its recombinant form a valuable tool for understanding disease mechanisms and developing targeted therapies. Ongoing research aims to clarify its dual roles in cancer and explore its potential as a diagnostic biomarker or therapeutic target.
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