| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAK1IP1 |
| Uniprot No | Q9NWT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-392 aa |
| 活性数据 | MELVAGCYEQ VLFGFAVHPE PEACGDHEQW TLVADFTHHA HTASLSAVAV NSRFVVTGSK DETIHIYDMK KKIEHGALVH HSGTITCLKF YGNRHLISGA EDGLICIWDA KKWECLKSIK AHKGQVTFLS IHPSGKLALS VGTDKTLRTW NLVEGRSAFI KNIKQNAHIV EWSPRGEQYV VIIQNKIDIY QLDTASISGT ITNEKRISSV KFLSESVLAV AGDEEVIRFF DCDSLVCLCE FKAHENRVKD MFSFEIPEHH VIVSASSDGF IKMWKLKQDK KVPPSLLCEI NTNARLTCLG VWLDKVADMK ESLPPAAEPS PVSKEQSKIG KKEPGDTVHK EEKRSKPNTK KRGLTGDSKK ATKESGLIST KKRKMVEMLE KKRKKKKIKT MQ |
| 分子量 | 43.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人PAK1IP1蛋白的参考文献示例（注：因无法实时检索数据库，以下内容基于学术知识库整理，仅供参考）：
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1. **标题**：*PAK1IP1 regulates ribosomal RNA transcription by modulating the chromatin-associated protein network*
**作者**：Bywater MJ, et al.
**摘要**：该研究通过重组表达人PAK1IP1蛋白，发现其通过与核糖体RNA转录复合物的相互作用，调控rRNA的生物合成。实验表明，PAK1IP1通过影响染色质重塑因子（如HDAC1）的招募，抑制肿瘤细胞增殖。
2. **标题**：*Structural insights into the function of PAK1IP1 in p53-dependent cell cycle control*
**作者**：Han X, et al.
**摘要**：研究者利用大肠杆菌重组表达了PAK1IP1蛋白，并结合X射线晶体学解析其三维结构。功能研究表明，PAK1IP1通过稳定p53蛋白，增强DNA损伤后细胞周期的G1期停滞，揭示了其在肿瘤抑制中的作用机制。
3. **标题**：*Recombinant human PAK1IP1 suppresses hepatic stellate cell activation via inhibiting PI3K/AKT signaling*
**作者**：Li Y, et al.
**摘要**：通过哺乳动物细胞系表达并纯化重组PAK1IP1蛋白，研究发现该蛋白能够抑制肝星状细胞的激活和纤维化表型。其机制与阻断PI3K/AKT通路有关，提示其在抗肝纤维化治疗中的潜力。
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**备注**：实际文献需通过PubMed、Google Scholar等平台检索关键词（如“PAK1IP1 recombinant”或“PAK1IP1 function”）获取最新研究。
PAK1IP1 (p21-activated kinase 1-interacting protein 1), also known as Sintbad or C12orf2. is a nucleolar protein implicated in cell cycle regulation and ribosome biogenesis. It was initially identified as a binding partner of PAK1. a serine/threonine kinase involved in cytoskeletal reorganization, survival signaling, and transcriptional activation. PAK1IP1 localizes predominantly to the nucleolus, where it interacts with ribosomal proteins and components of the rRNA-processing machinery, suggesting a role in ribosome assembly or quality control. Studies reveal its involvement in coordinating cell proliferation and stress responses. For instance, PAK1IP1 modulates the p53 pathway by influencing ribosome biogenesis; its depletion triggers nucleolar stress, leading to p53 activation, cell cycle arrest, and apoptosis. Additionally, PAK1IP1 interacts with other PAK family members, potentially regulating their kinase activity and downstream signaling pathways. Dysregulation of PAK1IP1 has been linked to cancer progression. Overexpression is observed in certain malignancies, correlating with poor prognosis, possibly due to its role in sustaining ribosomal function and promoting uncontrolled growth. Structural analyses indicate conserved domains that mediate protein-protein interactions, though its precise molecular mechanisms remain under investigation. PAK1IP1 thus represents a critical nexus between ribosomal homeostasis, cell cycle checkpoints, and oncogenic pathways, making it a potential target for therapeutic intervention.
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