关键信息
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基因名
ENO2
- 应用
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别名
ENO2；Gamma-enolase
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种属
Mouse
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P17183
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表达区间
1-434aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSMSIEKIWAREILDSRGNPTVEVDLYTA KGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGVLKAVDHINSRIAPAL ISSGISVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAGAAERD LPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAES FRDAMRLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELV KEAIDKAGYTEKMVIGMDVAASEFYRDGKYDLDFKSPADPSRYITGDQLG ALYQDFVRNYPVVSIEDPFDQDDWAAWSKFTANVGIQIVGDDLTVTNPKR IERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETE DTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHN FRNPSVL
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分子量
50 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ENO2, or enolase 2, is a glycolytic enzyme that plays a crucial role in cellular metabolism and energy production. It is primarily expressed in the brain and is considered a neuron-specific enolase, making it a potential biomarker for neurological diseases. Research on ENO2 has gained momentum due to its involvement in various pathological conditions, including neurodegenerative diseases, ischemic brain injury, and cancers. The protein exists in several isoforms, with ENO2 being distinctively associated with neuron function and development. Its overexpression has been linked to tumorigenesis, while its levels can serve as an indicator of neuronal damage, making it a target of intense study in both cancer and neuroscience fields. Additionally, ENO2 is being explored for its potential role in mediating neuronal signaling and plasticity, which are fundamental to cognitive processes. The recombinant production of ENO2 allows for detailed investigation into its structure-function relationships and interactions with other biomolecules, fostering insights that could lead to novel therapeutic strategies. As a versatile research tool, the availability of ENO2 as a recombinant protein supports advances in diagnostic applications and therapeutic interventions, highlighting its significance in both basic research and clinical settings. The ongoing studies aim not only to elucidate the biological functions of ENO2 but also to harness its potential for innovative approaches in treating neurodegenerative disorders and cancers, emphasizing the importance of this enzyme in health and disease.
