| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDPK2 |
| Uniprot No | O15530 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-556 aa |
| 活性数据 | MARTTSQLYD AVPIQSSVVL CSCPSPSMVR TQTESSTPPG IPGGSRQGPA MDGTAAEPRP GAGSLQHAQP PPQPRKKRPE DFKFGKILGE GSFSTVVLAR ELATSREYAI KILEKRHIIK ENKVPYVTRE RDVMSRLDHP FFVKLYFTFQ DDEKLYFGLS YAKNGELLKY IRKIGSFDET CTRFYTAEIV SALEYLHGKG IIHRDLKPEN ILLNEDMHIQ ITDFGTAKVL SPESKQARAN SFVGTAQYVS PELLTEKSAC KSSDLWALGC IIYQLVAGLP PFRAGNEYLI FQKIIKLEYD FPEKFFPKAR DLVEKLLVLD ATKRLGCEEM EGYGPLKAHP FFESVTWENL HQQTPPKLTA YLPAMSEDDE DCYGNYDNLL SQFGCMQVSS SSSSHSLSAS DTGLPQRSGS NIEQYIHDLD SNSFELDLQF SEDEKRLLLE KQAGGNPWHQ FVENNLILKM GPVDKRKGLF ARRRQLLLTE GPHLYYVDPV NKVLKGEIPW SQELRPEAKN FKTFFVHTPN RTYYLMDPSG NAHKWCRKIQ EVWRQRYQSH PDAAVQ |
| 分子量 | 63.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PDPK2(可能为 **PDK2** 或 **PDPK1** 的笔误，需确认名称准确性)的参考文献示例。若确为 **PDPK2**，可能存在文献名称或研究方向差异，以下内容基于假设性整理：
---
1. **文献名称**：*"Structural and Functional Characterization of Recombinant Human PDPK2: Implications for Kinase Activation"*
**作者**：Smith A, et al.
**摘要**：本文通过大肠杆菌系统成功表达并纯化重组人PDPK2蛋白，解析其三维结构，证明其对下游底物的磷酸化活性及在PI3K/AKT信号通路中的调控作用。
2. **文献名称**：*"PDPK2 Regulates Cellular Apoptosis in Cancer via Phosphorylation of FOXO Transcription Factors"*
**作者**：Chen L, et al.
**摘要**：研究揭示了重组人PDPK2在肿瘤细胞中通过磷酸化FOXO蛋白调控细胞凋亡的机制，为靶向PDPK2的抗癌治疗提供依据。
3. **文献名称**：*"Development of a High-Throughput Assay for Screening PDPK2 Inhibitors Using Recombinant Protein"*
**作者**：Kimura T, et al.
**摘要**：该研究利用重组人PDPK2蛋白建立高通量抑制剂筛选平台，鉴定出多种潜在小分子抑制剂，并验证其体外抑制活性。
4. **文献名称**：*"Expression and Purification of Recombinant Human PDPK2 in Mammalian Cells for Functional Studies"*
**作者**：Wang Y, et al.
**摘要**：通过哺乳动物表达系统制备重组人PDPK2蛋白，优化纯化条件，并验证其在胰岛素信号通路中对下游激酶的激活功能。
---
**注意**：以上文献为假设性示例。若需真实文献，请进一步确认 **PDPK2** 的命名准确性(如是否为 **PDK2** 或 **PDPK1**)，并提供具体研究方向(如结构、功能、疾病关联等)。建议通过PubMed或Web of Science检索关键字重组。
Recombinant human PDPK2 (3-phosphoinositide-dependent protein kinase 2), also known as PDK2. is a serine/threonine kinase critical in cellular energy metabolism and signal transduction. It regulates the pyruvate dehydrogenase complex (PDC) by phosphorylating and inactivating the E1 subunit, thereby modulating the conversion of pyruvate to acetyl-CoA—a key step linking glycolysis to the tricarboxylic acid (TCA) cycle. This control influences cellular energy homeostasis, particularly under conditions of abundant nutrients or low energy demand.
Produced via genetic engineering in expression systems like *E. coli* or mammalian cells, recombinant PDPK2 enables studies of its structure, enzymatic activity, and interaction with inhibitors. Its dysregulation is implicated in metabolic disorders, such as diabetes, obesity, and cancer, where altered glucose utilization supports tumor growth. Researchers leverage recombinant PDPK2 to screen therapeutic compounds targeting metabolic reprogramming in diseases. Additionally, structural analyses of the kinase domain aid in designing selective inhibitors. As a pivotal metabolic regulator, PDPK2 remains a promising focus for both basic research and drug development.
×
