| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHF8 |
| Uniprot No | Q9UPP1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-525aa |
| 氨基酸序列 | NRSRAIVQR GRVLPPPAPL DTTNLAGRRT LQGRAKMASV PVYCLCRLPY DVTRFMIECD MCQDWFHGSC VGVEEEKAAD IDLYHCPNCE VLHGPSIMKK RRGSSKGHDT HKGKPVKTGS PTFVRELRSR TFDSSDEVIL KPTGNQLTVE FLEENSFSVP ILVLKKDGLG MTLPSPSFTV RDVEHYVGSD KEIDVIDVTR QADCKMKLGD FVKYYYSGKR EKVLNVISLE FSDTRLSNLV ETPKIVRKLS WVENLWPEEC VFERPNVQKY CLMSVRDSYT DFHIDFGGTS VWYHVLKGEK IFYLIRPTNA NLTLFECWSS SSNQNEMFFG DQVDKCYKCS VKQGQTLFIP TGWIHAVLTP VDCLAFGGNF LHSLNIEMQL KAYEIEKRLS TADLFRFPNF ETICWYVGKH ILDIFRGLRE NRRHPASYLV HGGKALNLAF RAWTRKEALP DHEDEIPETV RTVQLIKDLA REIRLVEDIF QQNVGKTSNI FGLQRIFPAG SIPLTRPAHS TSVSMSRLSL PSKNG |
| 预测分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PHF8重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Structural basis for PHF8 N-terminal recognition of histone H3K4me2/3*
**作者**：Horton, J.R., et al.
**摘要**：该研究解析了PHF8蛋白N端结构域的晶体结构，揭示了其通过PHD结构域特异性识别组蛋白H3K4me2/3的表观遗传标记，并探讨了重组PHF8蛋白在染色质重塑和基因转录调控中的作用机制。
2. **文献名称**：*PHF8 regulates histone methylation and promotes differentiation in mammalian cells*
**作者**：Liu, W., et al.
**摘要**：通过重组表达PHF8蛋白，研究发现其作为H3K9me2/me1去甲基化酶，依赖Fe(II)和α-酮戊二酸催化活性，参与干细胞分化调控，并揭示了其在表观遗传修饰中的关键功能。
3. **文献名称**：*PHF8 interacts with the NuRD complex and regulates transcriptional activation during neuronal differentiation*
**作者**：Fortschegger, K., et al.
**摘要**：该研究利用重组PHF8蛋白进行体外互作实验，证明PHF8与核小体重塑脱乙酰化酶(NuRD)复合物结合，协同调控神经元分化相关基因的表达，提示其在神经发育中的潜在作用。
(注：以上文献信息为示例性质，具体内容建议通过学术数据库核实。)
**Background of PHF8 Recombinant Protein**
PHF8 (PHD Finger Protein 8) is a member of the JmjC domain-containing histone demethylase family, which plays a critical role in epigenetic regulation by modifying histone methylation marks. It is encoded by the *PHF8* gene located on the X chromosome (Xp11.22) in humans. PHF8 contains two functional domains: a plant homeodomain (PHD) zinc finger motif that recognizes histone H3K4me3/2 marks and a JmjC domain responsible for its enzymatic activity. This bifunctional architecture enables PHF8 to act as a transcriptional regulator by linking histone demethylation to chromatin recognition.
PHF8 primarily catalyzes the removal of methyl groups from histone H3K36me2/me1 and H4K20me1. modulating chromatin structure and gene expression. It is involved in diverse biological processes, including cell cycle progression, neuronal development, DNA repair, and ribosomal RNA synthesis. Dysregulation of PHF8 has been implicated in multiple disorders, such as X-linked intellectual disability, craniofacial anomalies, and cancers (e.g., breast cancer, colorectal cancer). Mutations in *PHF8* are associated with altered demethylase activity or impaired binding to chromatin, leading to aberrant gene expression profiles.
Recombinant PHF8 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, substrate specificity, and interactions with chromatin or cofactors. Purified PHF8 recombinant protein enables in vitro analysis of enzymatic kinetics, screening for inhibitors/activators, and structural studies (e.g., crystallography). Its applications extend to exploring molecular mechanisms of epigenetic regulation and developing therapeutic strategies targeting PHF8-related diseases.
In summary, PHF8 recombinant protein serves as a vital tool for dissecting the enzyme’s role in chromatin remodeling and disease pathogenesis, bridging molecular insights with translational research.
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