| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRIN2C |
| Uniprot No | Q14957 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1233aa |
| 氨基酸序列 | MGGALGPALLLTSLFGAWAGLGPGQGEQGMTVAVVFSSSGPPQAQFRARLTPQSFLDLPLEIQPLTVGVNTTNPSSLLTQICGLLGAAHVHGIVFEDNVDTEAVAQILDFISSQTHVPILSISGGSAVVLTPKEPGSAFLQLGVSLEQQLQVLFKVLEEYDWSAFAVITSLHPGHALFLEGVRAVADASHVSWRLLDVVTLELGPGGPRARTQRLLRQLDAPVFVAYCSREEAEVLFAEAAQAGLVGPGHVWLVPNLALGSTDAPPATFPVGLISVVTESWRLSLRQKVRDGVAILALGAHSYWRQHGTLPAPAGDCRVHPGPVSPAREAFYRHLLNVTWEGRDFSFSPGGYLVQPTMVVIALNRHRLWEMVGRWEHGVLYMKYPVWPRYSASLQPVVDSRHLTVATLEERPFVIVESPDPGTGGCVPNTVPCRRQSNHTFSSGDVAPYTKLCCKGFCIDILKKLARVVKFSYDLYLVTNGKHGKRVRGVWNGMIGEVYYKRADMAIGSLTINEERSEIVDFSVPFVETGISVMVARSNGTVSPSAFLEPYSPAVWVMMFVMCLTVVAITVFMFEYFSPVSYNQNLTRGKKSGGPAFTIGKSVWLLWALVFNNSVPIENPRGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEQYIDTVSGLSDKKFQRPQDQYPPFRFGTVPNGSTERNIRSNYRDMHTHMVKFNQRSVEDALTSLKMGKLDAFIYDAAVLNYMAGKDEGCKLVTIGSGKVFATTGYGIAMQKDSHWKRAIDLALLQFLGDGETQKLETVWLSGICQNEKNEVMSSKLDIDNMAGVFYMLLVAMGLALLVFAWEHLVYWKLRHSVPNSSQLDFLLAFSRGIYSCFSGVQSLASPPRQASPDLTASSAQASVLKMLQAARDMVTTAGVSSSLDRATRTIENWGGGRRAPPPSPCPTPRSGPSPCLPTPDPPPEPSPTGWGPPDGGRAALVRRAPQPPGRPPTPGPPLSDVSRVSRRPAWEARWPVRTGHCGRHLSASERPLSPARCHYSSFPRADRSGRPFLPLFPELEDLPLLGPEQLARREALLHAAWARGSRPRHASLPSSVAEAFARPSSLPAGCTGPACARPDGHSACRRLAQAQSMCLPIYREACQEGEQAGAPAWQHRQHVCLHAHAHLPFCWGAVCPHLPPCASHGSWLSGAWGPLGHRGRTLGLGTGYRDSGGLDEISRVARGTQGFPGPCTWRRISSLESEV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GRIN2C重组蛋白的3篇文献示例(虚构内容，供参考格式)：
1. **文献名称**: "Functional Expression and Purification of Recombinant GRIN2C in HEK293 Cells"
**作者**: Smith A, et al.
**摘要**: 本研究优化了GRIN2C与GRIN1亚基在HEK293细胞中的共表达条件，纯化得到功能性NMDA受体复合物，并验证其谷氨酸和甘氨酸依赖的离子通道活性。
2. **文献名称**: "Cryo-EM Structure of the GRIN2C-Containing NMDA Receptor in a Lipid Bilayer"
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜解析GRIN2C/GluN2C重组受体在脂质双层中的三维结构，揭示其独特的亚基界面构象及变构调节位点，为靶向药物设计提供依据。
3. **文献名称**: "Pharmacological Profiling of GRIN2C Recombinant Receptors Using Patch-Clamp Electrophysiology"
**作者**: Tanaka K, et al.
**摘要**: 系统测试了多种NMDA受体拮抗剂(如MK-801、ifenprodil)对重组GRIN2C受体的抑制作用，发现其药理学响应与GRIN2A/B亚型存在显著差异。
*注：以上文献为模拟示例，实际研究中建议通过PubMed或Web of Science检索真实发表的论文。*
**Background of GRIN2C Recombinant Protein**
GRIN2C, also known as glutamate ionotropic receptor NMDA type subunit 2C, is a critical component of N-methyl-D-aspartate (NMDA) receptors, a subclass of ionotropic glutamate receptors central to synaptic plasticity, learning, and memory. Encoded by the *GRIN2C* gene in humans (located on chromosome 17q25), the GRIN2C protein forms heterotetrameric complexes with GRIN1 (and occasionally GRIN2A/B/D subunits) to assemble functional NMDA receptors. These ligand-gated channels require dual agonists (glutamate and glycine) for activation and are permeable to calcium, playing pivotal roles in excitatory neurotransmission and neuronal development.
GRIN2C-containing NMDA receptors exhibit distinct biophysical properties, including reduced magnesium sensitivity and slower kinetics compared to other GRIN2 subunits. This subunit is predominantly expressed in the cerebellum, thalamus, and olfactory regions, suggesting specialized roles in motor coordination, sensory processing, and cognitive functions. Dysregulation of GRIN2C has been implicated in neurological disorders such as epilepsy, schizophrenia, and Alzheimer’s disease, making it a target for therapeutic research.
Recombinant GRIN2C proteins are engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to study receptor structure-function relationships, screen pharmacological compounds, or investigate disease mechanisms. These proteins often include tags (e.g., His-tag, FLAG) for purification and detection. By enabling precise control over subunit composition and post-translational modifications, recombinant GRIN2C facilitates in vitro studies on receptor assembly, ligand binding, and allosteric modulation, advancing drug discovery and mechanistic neuroscience.
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