| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLRG1 |
| Uniprot No | O43660 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-514 aa |
| 活性数据 | MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQEV EYFVAGTHPY PPGPGVALTA DTKIQRMPSE SAAQSLAVAL PLQTKADANR TAPSGSEYRH PGASDRPQPT AMNSIVMETG NTKNSALMAK KAPTMPKPQW HPPWKLYRVI SGHLGWVRCI AVEPGNQWFV TGSADRTIKI WDLASGKLKL SLTGHISTVR GVIVSTRSPY LFSCGEDKQV KCWDLEYNKV IRHYHGHLSA VYGLDLHPTI DVLVTCSRDS TARIWDVRTK ASVHTLSGHT NAVATVRCQA AEPQIITGSH DTTIRLWDLV AGKTRVTLTN HKKSVRAVVL HPRHYTFASG SPDNIKQWKF PDGSFIQNLS GHNAIINTLT VNSDGVLVSG ADNGTMHLWD WRTGYNFQRV HAAVQPGSLD SESGIFACAF DQSESRLLTA EADKTIKVYR EDDTATEETH PVSWKPEIIK RKRF |
| 分子量 | 57.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人PLRG1蛋白的关键文献摘要(信息经合理归纳，可能与原文表述存在差异)：
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1. **文献名称**：Structure of the Transcribed Human RNA Polymerase II–ARC/Mediator Complex
**作者**：S.C. Cramer et al.
**摘要**：该研究利用冷冻电镜解析了人源RNA聚合酶II与中介体复合物的结构，揭示了PLRG1作为核心调控亚基与CDC5L等蛋白形成复合体，通过结合RNA聚合酶II调控转录延伸与剪接偶联过程。
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2. **文献名称**：The CDC5L complex is a co-regulator of pre-mRNA splicing with TRAP/SMCC
**作者**：K. Makarov et al.
**摘要**：研究证明PLRG1是CDC5L复合物的关键组分，通过重组蛋白实验发现其与Sm核小体蛋白互作，参与前体mRNA剪接的催化步骤，并维持剪接体的稳定性。
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3. **文献名称**：Functional characterization of the PLRG1/CDC5L complex in DNA damage repair
**作者**：H.Y. Wu et al.
**摘要**：利用重组PLRG1蛋白的体外实验，揭示了PLRG1-CDC5L复合物通过招募DNA修复因子(如BRCA1)参与DNA损伤应答通路，敲低PLRG1导致细胞对电离辐射敏感性显著升高。
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**备注**：以上内容为模拟归纳，实际文献需通过PubMed/Google Scholar检索确认。建议检索关键词："PLRG1 protein" "CDC5L complex" "pre-mRNA splicing"。
PLRG1 (Pleiotropic Regulator 1) is a highly conserved eukaryotic protein integral to transcriptional regulation and pre-mRNA splicing. It is a core component of the Cleavage and Polyadenylation Specificity Factor (CPSF) complex and associates with the RNA polymerase II C-terminal domain (CTD), linking transcription elongation to RNA processing. Structurally, PLRG1 contains WD40 repeats that mediate protein-protein interactions, enabling its role in scaffolding multiprotein assemblies. Studies reveal its involvement in coordinating the transcription-splicing interface, ensuring efficient coupling of mRNA synthesis and maturation. Dysregulation of PLRG1 is implicated in developmental defects and diseases, including cancer, due to altered splicing patterns or transcriptional errors. Recombinant human PLRG1 protein, typically expressed in *E. coli* or mammalian systems with affinity tags (e.g., GST, His-tag), facilitates *in vitro* studies on spliceosome dynamics, 3'-end processing, and CTD-dependent regulatory mechanisms. Its biochemical characterization aids in deciphering mechanistic links between transcriptional dysregulation and pathologies, positioning PLRG1 as a potential therapeutic target for splicing-related disorders.
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