| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PORCN |
| Uniprot No | Q9H237 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-461 aa |
| 活性数据 | MATFSRQEFFQQLLQGCLLPTAQQGLDQIWLLLAICLACRLLWRLGLPSYLKHASTVAGGFFSLYHFFQLHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGEVGTVPSPVEFMGYLYFVGTIVFGPWISFHSYLQAVQGRPLSCRWLQKVARSLALALLCLVLSTCVGPYLFPYFIPLNGDRLLRNKKRKARGTMVRWLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEEKDHLEWDLTVSKPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAVLLSLAFITYVEHVLRKRLARILSACVLSKRCPPDCSHQHRLGLGVRALNLLFGALAIFHLAYLGSLFDVDVDDTTEEQGYGMAYTVHKWSELSWASHWVTFGCWIFYRLIG |
| 分子量 | 53.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PORCN蛋白的3篇参考文献，简要概括如下：
1. **"Structural basis of Wnt recognition by Frizzled"**
*作者：Janda et al.*
摘要：解析了人源PORCN介导Wnt蛋白棕榈酰化的分子机制，并利用重组PORCN蛋白阐明其催化结构域对Wnt信号分泌的关键作用。
2. **"PORCN inhibitors target Wnt-driven cancers through selective suppression of ligand production"**
*作者：Liu et al.*
摘要：研究开发基于重组PORCN蛋白活性筛选的小分子抑制剂，证明其通过抑制Wnt蛋白修饰有效抑制多种癌症模型生长。
3. **"Recombinant expression and functional characterization of human PORCN in lipid metabolism"**
*作者：Zhang & Chen*
摘要：报道了在昆虫细胞系统中高效表达功能性重组人PORCN蛋白，并验证其棕榈酰转移酶活性及对细胞脂代谢通路的影响。
(注：以上为示例性内容，实际文献请通过学术数据库检索。)
PORCN (Porcupine homolog) is an endoplasmic reticulum-localized membrane-bound O-acyltransferase essential for post-translational modification of Wnt proteins. It catalyzes the addition of palmitoleic acid to Wnts, a lipid modification critical for their proper secretion, stability, and interaction with cell surface receptors. As a key regulator of Wnt signaling, PORCN plays pivotal roles in embryonic development, tissue homeostasis, and stem cell maintenance. Dysregulation of PORCN-mediated Wnt processing is implicated in cancers (e.g., colorectal, breast) and developmental disorders like Goltz syndrome (focal dermal hypoplasia), caused by X-linked PORCN mutations.
Recombinant human PORCN protein, typically produced in mammalian or insect cell systems, retains the catalytic domain structure with multiple transmembrane regions. It serves as a vital tool for studying Wnt biogenesis, molecular interactions, and inhibitor screening. Researchers utilize it to develop PORCN inhibitors (e.g., LGK974) that block Wnt secretion in cancers dependent on autocrine Wnt signaling. Structural studies using recombinant PORCN have revealed insights into its hydrophobic substrate-binding channel and acylation mechanisms. Current applications extend to organoid culture optimization, where PORCN activity modulates Wnt gradient formation for tissue modeling. Its status as a druggable target continues to drive pharmaceutical interest in modulating Wnt pathways for therapeutic interventions.
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