关键信息
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基因名
omcB
- 应用
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别名
omcB；omp2；omp2B；Large cysteine-rich periplasmic Protein OmcB
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P23700
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表达区间
41-196aa
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氨基酸序列
SAETKPAPVPMTAKKVRLVRRNKQPVEQKSRGAFCDKEFYPCEEGRCQPVEAQQESCYGRLYSVKVNDDCNVEICQSVPEYATVGSPYPIEILAIGKKDCVDVVITQQLPCEAEFVSSDPETTPTSDGKLVWKIDRLGAGDKCKITVWVKPLKEGC
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分子量
24.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
OmcB, an outer membrane cytochrome, plays a crucial role in the electron transfer processes of certain bacteria, particularly Geobacter species, which are known for their unique ability to transfer electrons to solid substrates during anaerobic respiration. This characteristic makes OmcB a significant subject of study in bioenergy and bioremediation applications, as these bacteria can be harnessed for microbial fuel cells or in the cleanup of contaminated environments. Research has shown that OmcB is essential for the reduction of metal oxides and supports the extracellular electron transfer (EET) mechanisms that enable these microorganisms to survive in low-energy environments. The structural and functional characterization of recombinant OmcB provides insights into its electron transfer capabilities and interactions with other cellular components. These studies are instrumental in elucidating the molecular mechanisms underlying extracellular electron transfer, contributing to advancements in biotechnology and environmental engineering. Understanding the properties of recombinant OmcB also opens avenues for developing novel biotechnological applications, such as biosensors or enhanced bioremediation strategies, thereby highlighting the potential of manipulating microbial processes for sustainable energy and environmental solutions.
