| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R1B |
| Uniprot No | P30154 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-601 aa |
| 活性数据 | AGASELGTG PGAAGGDGDD SLYPIAVLID ELRNEDVQLR LNSIKKLSTI ALALGVERTR SELLPFLTDT IYDEDEVLLA LAEQLGNFTG LVGGPDFAHC LLPPLENLAT VEETVVRDKA VESLRQISQE HTPVALEAYF VPLVKRLASG DWFTSRTSAC GLFSVCYPRA SNAVKAEIRQ QFRSLCSDDT PMVRRAAASK LGEFAKVLEL DSVKSEIVPL FTSLASDEQD SVRLLAVEAC VSIAQLLSQD DLETLVMPTL RQAAEDKSWR VRYMVADRFS ELQKAMGPKI TLNDLIPAFQ NLLKDCEAEV RAAAAHKVKE LGENLPIEDR ETIIMNQILP YIKELVSDTN QHVKSALASV IMGLSTILGK ENTIEHLLPL FLAQLKDECP DVRLNIISNL DCVNEVIGIR QLSQSLLPAI VELAEDAKWR VRLAIIEYMP LLAGQLGVEF FDEKLNSLCM AWLVDHVYAI REAATNNLMK LVQKFGTEWA QNTIVPKVLV MANDPNYLHR MTTLFCINAL SEACGQEITT KQMLPIVLKM AGDQVANVRF NVAKSLQKIG PILDTNALQG EVKPVLQKLG QDEDMDVKYF AQEAISVLAL A |
| 分子量 | 66.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人PPP2R1B蛋白的参考文献摘要示例：
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1. **文献名称**: "Protein phosphatase 2A regulatory subunit B56β modulates cancer cell survival and tumorigenesis"
**作者**: Wang SS, et al.
**摘要**: 本研究通过重组PPP2R1B蛋白表达，揭示了其在肺癌细胞中作为肿瘤抑制因子的功能。实验表明，PPP2R1B缺失导致PI3K/AKT信号通路异常激活，促进细胞增殖和转移。
2. **文献名称**: "Structural basis of PP2A regulation by B56 family subunits"
**作者**: Ruediger R, et al.
**摘要**: 利用重组PPP2R1B蛋白进行结构解析，阐明了其作为PP2A调节亚基如何结合核心酶并调控底物特异性，揭示了B56β亚基在细胞周期检查点中的分子机制。
3. **文献名称**: "PPP2R1B mutations drive resistance to HER2-targeted therapies in breast cancer"
**作者**: Masters TA, et al.
**摘要**: 研究发现重组PPP2R1B蛋白功能缺失会干扰DNA损伤修复通路，导致乳腺癌细胞对曲妥珠单抗耐药，提示其在治疗应答中的潜在生物标志物作用。
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**注**：上述文献为示例，实际引用需根据具体研究内容查询PubMed、Google Scholar等数据库获取准确信息。
Recombinant human PPP2R1B protein, also known as Protein Phosphatase 2 Scaffold Subunit Aβ, is a key regulatory component of the protein phosphatase 2A (PP2A) complex, a serine/threonine phosphatase critical for diverse cellular processes. PP2A is involved in signal transduction, cell cycle regulation, apoptosis, and metabolism. The PPP2R1B gene encodes the Aβ subunit, a structural scaffold that stabilizes the PP2A holoenzyme by linking the catalytic C subunit and regulatory B subunits. This architecture allows PP2A to adopt distinct functional forms, modulating substrate specificity and localization.
PPP2R1B has been implicated in tumor suppression, as its gene is frequently deleted or epigenetically silenced in cancers, including lung, colorectal, and breast cancers. Loss of PPP2R1B disrupts PP2A-mediated dephosphorylation of oncogenic signaling molecules (e.g., Akt, c-Myc), promoting uncontrolled cell proliferation and survival. Recombinant PPP2R1B is produced using expression systems (e.g., E. coli, mammalian cells) for functional studies, structural analysis, or screening therapeutic agents targeting PP2A dysfunction. Its applications span cancer research, neurodegenerative disease studies (PP2A dysfunction is linked to tau hyperphosphorylation in Alzheimer’s), and drug development to restore PP2A activity. Research on recombinant PPP2R1B enhances understanding of PP2A’s role in cellular homeostasis and disease mechanisms.
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