| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRPSAP1 |
| Uniprot No | Q14558 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-356 aa |
| 活性数据 | MNAARTGYRV FSANSTAACT ELAKRITERL GAELGKSVVY QETNGETRVE IKESVRGQDI FIIQTIPRDV NTAVMELLIM AYALKTACAR NIIGVIPYFP YSKQSKMRKR GSIVCKLLAS MLAKAGLTHI ITMDLHQKEI QGFFSFPVDN LRASPFLLQY IQEEIPNYRN AVIVAKSPDA AKRAQSYAER LRLGLAVIHG EAQCTELDMD DGRHSPPMVK NATVHPGLEL PLMMAKEKPP ITVVGDVGGR IAIIVDDIID DVESFVAAAE ILKERGAYKI YVMATHGILS AEAPRLIEES SVDEVVVTNT VPHEVQKLQC PKIKTVDISL ILSEAIRRIH NGESMAYLFR NITVDD |
| 分子量 | 39.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人PRPSAP1蛋白相关的参考文献(注：以下内容为示例性虚构文献，实际研究中建议通过学术数据库检索)：
1. **文献名称**: *Expression and Functional Analysis of Recombinant PRPSAP1 in Hepatocellular Carcinoma*
**作者**: Li, X. et al.
**摘要**: 研究利用大肠杆菌系统成功表达重组人PRPSAP1蛋白，并验证其在肝癌细胞中通过调节嘌呤代谢促进肿瘤增殖的功能。实验表明PRPSAP1缺失会显著抑制癌细胞生长。
2. **文献名称**: *PRPSAP1 Interacts with c-MYC to Modulate Cellular Energy Metabolism*
**作者**: Zhang, Y. et al.
**摘要**: 通过免疫共沉淀和重组PRPSAP1蛋白体外实验，揭示PRPSAP1与c-MYC蛋白结合，共同调控糖酵解途径，影响肿瘤细胞的能量代谢和存活。
3. **文献名称**: *Structural Basis of PRPSAP1 in Activating PRPS1 for De Novo Purine Synthesis*
**作者**: Wang, T. et al.
**摘要**: 通过重组PRPSAP1与PRPS1蛋白的共结晶实验，阐明PRPSAP1通过特定结构域增强PRPS1酶的活性，促进嘌呤核苷酸合成，为相关代谢疾病提供分子机制。
提示：实际文献可通过PubMed、Google Scholar等平台检索关键词“recombinant PRPSAP1”或“PRPSAP1 protein function”获取。
**Background of Recombinant Human PRPSAP1 Protein**
Phosphoribosyl pyrophosphate synthetase-associated protein 1 (PRPSAP1) is a human protein encoded by the *PRPSAP1* gene, belonging to the PRPSAP family. It interacts with phosphoribosyl pyrophosphate synthetase (PRPS), a key enzyme in nucleotide biosynthesis, regulating the *de novo* purine and pyrimidine pathways. PRPSAP1 is proposed to modulate PRPS activity or stability, influencing cellular pools of PRPP (5-phosphoribosyl-1-pyrophosphate), a critical metabolite for nucleotide synthesis and salvage pathways. Dysregulation of PRPSAP1 has been linked to metabolic disorders, mitochondrial dysfunction, and cancers, with studies suggesting its role in tumor proliferation and chemoresistance.
Recombinant human PRPSAP1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. It retains biological activity, enabling studies on its molecular interactions, enzymatic regulation, and mechanisms in disease contexts. This recombinant tool aids in elucidating PRPSAP1’s structural domains, post-translational modifications, and its crosstalk with metabolic or signaling pathways. Additionally, it supports drug discovery targeting nucleotide metabolism aberrations, particularly in oncology. Current research focuses on understanding PRPSAP1’s tissue-specific roles and its potential as a biomarker or therapeutic target in metabolic syndromes and malignancies.
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