关键信息
-
基因名
eutC
- 应用
-
别名
eutC；Ethanolamine ammonia-lyase small subunit
-
种属
E.coli
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
P19636
-
表达区间
1-295aa
-
氨基酸序列
MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCALDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR
-
分子量
35.8 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
EutC, a key enzyme in the ethanolamine utilization (Eut) pathway, plays a pivotal role in the metabolism of ethanolamine, which is an important nitrogen source for various microorganisms. Research on EutC is significant due to its involvement in microbial growth and the potential applications in biotechnology and environmental science. Understanding the structure and function of EutC can provide insights into bacterial adaptation mechanisms in nutrient-limited environments. Additionally, studying EutC can lead to advancements in synthetic biology, where engineered microorganisms could be designed for bioremediation or biofuel production using ethanolamine as a substrate. The recombinant production of EutC protein in heterologous systems, such as E. coli, allows for the detailed analysis of its biochemical properties and catalytic mechanisms. Approaches like site-directed mutagenesis and structural biology techniques, including X-ray crystallography and NMR spectroscopy, can unravel its active site configuration and interaction with substrates. As the demand for sustainable processes grows, EutC's potential role in metabolic engineering and its applications in biotechnological innovations underscore the importance of this research area in microbiology and environmental biotechnology.
