| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTGS1 |
| Uniprot No | P23219 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-599 aa |
| 活性数据 | ADPGAPTPVNPCCYYPCQHQGICVRFGLDRYQCDCTRTGYSGPNCTIPGLWTWLRNSLRPSPSFTHFLLTHGRWFWEFVNATFIREMLMRLVLTVRSNLIPSPPTYNSAHDYISWESFSNVSYYTRILPSVPKDCPTPMGTKGKKQLPDAQLLARRFLLRRKFIPDPQGTNLMFAFFAQHFTHQFFKTSGKMGPGFTKALGHGVDLGHIYGDNLERQYQLRLFKDGKLKYQVLDGEMYPPSVEEAPVLMHYPRGIPPQSQMAVGQEVFGLLPGLMLYATLWLREHNRVCDLLKAEHPTWGDEQLFQTTRLILIGETIKIVIEEYVQQLSGYFLQLKFDPELLFGVQFQYRNRIAMEFNHLYHWHPLMPDSFKVGSQEYSYEQFLFNTSMLVDYGVEALVDAFSRQIAGRIGGGRNMDHHILHVAVDVIRESREMRLQPFNEYRKRFGMKPYTSFQELVGEKEMAAELEELYGDIDALEFYPGLLLEKCHPNSIFGESMIEIGAPFSLKGLLGNPICSPEYWKPSTFGGEVGFNIVKTATLKKLVCLNTKTCPYVSFRVPDASQDDGPAVERPSTEL |
| 分子量 | 71 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PTGS1(COX-1)蛋白的3篇模拟参考文献示例：
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1. **标题**：*Prokaryotic Expression and Functional Characterization of Human PTGS1 for Structural Studies*
**作者**：Smith J, et al.
**摘要**：研究成功在大肠杆菌中表达了可溶性重组人PTGS1蛋白，通过亲和层析纯化后，利用体外酶活实验证实其催化花生四烯酸转化为前列腺素H2的能力，为后续晶体结构解析奠定基础。
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2. **标题**：*Crystal Structure of Recombinant Human PTGS1 in Complex with Aspirin*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析了重组PTGS1与阿司匹林结合的复合物结构，揭示了阿司匹林通过乙酰化Ser530不可逆抑制COX-1活性的分子机制，为非甾体抗炎药(NSAIDs)的优化设计提供了结构依据。
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3. **标题**：*High-Throughput Screening of COX-1 Inhibitors Using Recombinant PTGS1 Protein*
**作者**：Brown R, et al.
**摘要**：基于重组PTGS1蛋白开发了一种高通量筛选平台，用于快速评估化合物库对COX-1活性的抑制作用，发现了多个具有潜在心血管保护作用的天然产物抑制剂。
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*注：以上为模拟参考文献，实际研究中需以真实发表的文献为准。*
Prostaglandin-endoperoxide synthase 1 (PTGS1), also known as cyclooxygenase-1 (COX-1), is a constitutively expressed enzyme critical for prostanoid biosynthesis. It catalyzes the conversion of arachidonic acid into prostaglandin H2 (PGH2), a precursor for various prostaglandins and thromboxanes involved in inflammation, pain response, fever, and homeostasis. Unlike its inducible isoform PTGS2/COX-2. PTGS1 is ubiquitously expressed in most tissues under basal conditions, playing essential roles in gastrointestinal mucosal protection, platelet aggregation, and renal function.
Recombinant human PTGS1 protein is produced via genetic engineering in heterologous expression systems (e.g., insect or mammalian cells) to study its structure, enzymatic activity, and regulatory mechanisms. Its recombinant form retains the bifunctional activity of cyclooxygenase and peroxidase, enabling research into lipid metabolism, NSAID (non-steroidal anti-inflammatory drug) interactions, and pathological conditions like cardiovascular diseases or cancer. PTGS1 is a primary target of aspirin, which irreversibly inhibits its enzymatic activity to reduce thrombotic risk. Structural studies of recombinant PTGS1 have revealed key domains for substrate binding and inhibitor selectivity, aiding the development of isoform-specific therapeutics. Ongoing research focuses on its role in cellular signaling beyond inflammation, including tissue repair and angiogenesis.
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