| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SP140 |
| Uniprot No | Q13342 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-867aa |
| 氨基酸序列 | MAQQGQQGQMASGDSNLNFRMVAEIQNVEGQNLQEQVCPEPIFRFFRENKVEIASAITRPFPFLMGLRDRSFISEQMYEHFQEAFRNLVPVTRVMYCVLSELEKTFGWSHLEALFSRINLMAYPDLNEIYRSFQNVCYEHSPLQMNNVNDLEDRPRLLPYGKQENSNACHEMDDIAVPQEALSSSPRCEPGFSSESCEQLALPKAGGGDAEDAPSLLPGGGVSCKLAIQIDEGESEEMPKLLPYDTEVLESNGMIDAARTYSTAPGEKQGEEEGRNSPRKRNQDKEKYQESPEGRDKETFDLKTPQVTNEGEPEKGLCLLPGEGEEGSDDCSEMCDGEEPQEASSSLARCGSVSCLSAETFDLKTPQVTNEGEPEKELSLLPGEGEEGSDDCSEMCDGEERQEASSSLARRGSVSSELENHPMNEEGESEELASSLLYDNVPGAEQSAYENEKCSCVMCFSEEVPGSPEARTESDQACGTMDTVDIANNSTLGKPKRKRRKKRGHGWSRMRMRRQENSQQNDNSKADGQVVSSEKKANVNLKDLSKIRGRKRGKPGTRFTQSDRAAQKRVRSRASRKHKDETVDFKAPLLPVTCGGVKGILHKKKLQQGILVKCIQTEDGKWFTPTEFEIKGGHARSKNWRLSVRCGGWPLRWLMENGFLPDPPRIRYRKKKRILKSQNNSSVDPCMRNLDECEVCRDGGELFCCDTCSRVFHEDCHIPPVEAERTPWNCIFCRMKESPGSQQCCQESEVLERQMCPEEQLKCEFLLLKVYCCSESSFFAKIPYYYYIREACQGLKEPMWLDKIKKRLNEHGYPQVEGFVQDMRLIFQNHRASYKYKDFGQMGFRLEAEFEKNFKEVFAIQETNGNN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SP140重组蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*SP140 is a transcriptional regulator in human macrophages with a role in interferon signaling*
**作者**：Jeffrey A. Silverman et al.
**摘要**：该研究揭示了SP140蛋白在巨噬细胞中的转录调控作用，发现其通过表观遗传修饰调控炎症相关基因。重组SP140蛋白实验表明其能抑制促炎细胞因子表达，提示其在免疫调节中的潜在应用。
2. **文献名称**：*Interferon-inducible SP140 mediates antiviral immunity by targeting viral ribonucleoproteins*
**作者**：Hui Zhang et al.
**摘要**：研究证明，SP140是干扰素诱导的抗病毒蛋白，重组SP140通过结合病毒RNPs(如流感病毒RNA)抑制复制。结构分析显示其N端SAND结构域对病毒RNA识别至关重要。
3. **文献名称**：*Structural and functional analysis of the SP140 protein in autoimmune susceptibility*
**作者**：Maria J. Lewis et al.
**摘要**：该研究通过重组SP140蛋白解析其晶体结构，发现特定结构域(如PHD锌指)的突变与克罗恩病风险相关。功能实验表明突变体影响核小体结合能力，为自身免疫病机制提供新见解。
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**备注**：若需获取具体文献，建议在PubMed或Google Scholar以关键词“SP140 recombinant protein”检索，并筛选近5年研究以获取最新进展。部分研究可能涉及SP140的基因调控机制，需注意区分重组蛋白的直接应用。
SP140 (Speckled Protein 140) is a nuclear protein encoded by the *SP140* gene, initially identified for its association with autoimmune and inflammatory diseases. It belongs to the SP100 family of proteins, characterized by the presence of conserved structural domains such as SAND, PHD, and bromodomains, which mediate chromatin interactions and epigenetic regulation. SP140 is predominantly expressed in immune cells, including macrophages, dendritic cells, and B lymphocytes, where it localizes to nuclear speckles and plays a role in transcriptional regulation, chromatin remodeling, and innate immune responses. Studies link SP140 to the modulation of inflammatory gene expression, particularly through interactions with histone deacetylases (HDACs) and other chromatin-modifying enzymes.
Recombinant SP140 protein is engineered to facilitate functional and structural studies. Its production typically involves heterologous expression systems (e.g., *E. coli* or mammalian cells) to generate tagged or untagged versions for experimental use. Researchers employ recombinant SP140 to investigate its role in immune signaling pathways, such as NF-κB and interferon responses, and its dysregulation in diseases like Crohn’s disease, primary biliary cholangitis, and chronic lymphocytic leukemia. Notably, polymorphisms in the *SP140* gene are associated with altered protein function and disease susceptibility, highlighting its clinical relevance. Recombinant SP140 also serves as a tool for drug discovery, enabling screening for small molecules targeting its chromatin-binding domains to modulate immune activity. Overall, SP140 represents a critical nexus between epigenetic regulation and immune homeostasis, with recombinant forms accelerating mechanistic and therapeutic exploration.
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