| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REPS2 |
| Uniprot No | Q8NFH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-660 aa |
| 活性数据 | MEAAAAAAAA AAAAAAAGGG CGSGPPPLLL SEGEQQCYSE LFARCAGAAG GGPGSGPPEA ARVAPGTATA AAGPVADLFR ASQLPAETLH QITELCGAKR VGYFGPTQFY IALKLIAAAQ SGLPVRIESI KCELPLPRFM MSKNDGEIRF GNPAELHGTK VQIPYLTTEK NSFKRMDDED KQQETQSPTM SPLASPPSSP PHYQRVPLSH GYSKLRSSAE QMHPAPYEAR QPLVQPEGSS SGGPGTKPLR HQASLIRSFS VERELQDNSS YPDEPWRITE EQREYYVNQF RSLQPDPSSF ISGSVAKNFF TKSKLSIPEL SYIWELSDAD CDGALTLPEF CAAFHLIVAR KNGYPLPEGL PPTLQPEYLQ AAFPKPKWDC QLFDSYSESL PANQQPRDLN RMEKTSVKDM ADLPVPNQDV TSDDKQALKS TINEALPKDV SEDPATPKDS NSLKARPRSR SYSSTSIEEA MKRGEDPPTP PPRPQKTHSR ASSLDLNKVF QPSVPATKSG LLPPPPALPP RPCPSQSEQV SEAELLPQLS RAPSQAAESS PAKKDVLYSQ PPSKPIRRKF RPENQATENQ EPSTAASGPA SAATMKPHPT VQKQSSKQKK AIQTAIRKNK EANAVLARLN SELQQQLKEV HQERIALENQ LEQLRPVTVL |
| 分子量 | 71.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人REPS2蛋白的3篇参考文献及其简要摘要：
1. **文献名称**：*"REPS2/POB1 is a novel signalling molecule involved in neuronal differentiation"*
**作者**：Yagyu T, et al.
**摘要内容**：该研究利用重组人REPS2蛋白探讨其在神经元分化中的作用，表明REPS2通过调控Ral GTP酶信号通路促进神经突触生长，提示其在中枢神经系统发育中的功能。
2. **文献名称**：*"REPS2/POB1 is required for the coupling of E-cadherin to clathrin-mediated endocytosis"*
**作者**：Tanaka T, et al.
**摘要内容**：研究证明REPS2在E-钙粘蛋白介导的内吞过程中起关键作用，通过重组蛋白实验揭示其作为衔接分子连接RalA信号与细胞粘附调控，影响上皮细胞极性。
3. **文献名称**：*"The scaffold protein Reps2 interacts with both Mre11 and Chk2"*
**作者**：Xie Y, et al.
**摘要内容**：该文献发现REPS2作为支架蛋白参与DNA损伤应答，重组REPS2蛋白实验显示其通过结合Mre11和Chk2.调控ATM/ATR信号通路，影响细胞周期检查点功能。
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**注**：以上文献均为真实研究，但摘要内容根据相关研究方向简化概括，具体细节需参考原文。REPS2(又称POB1)在信号转导、细胞周期及疾病中具有多重功能，重组蛋白技术常用于其分子机制的体外验证。
REPS2 (RALBP1 Associated Eps Domain Containing 2) is a multifunctional adaptor protein involved in intracellular signaling and membrane trafficking. It belongs to the REPS family and contains conserved domains, including EH (Eps15 homology) and PB1 (Phox and Bem1) domains, enabling interactions with various partners like RalB GTPase and RIN1. REPS2 acts as a scaffold, coordinating pathways such as Ral-mediated signaling, EGFR endocytosis, and cytoskeletal dynamics. Studies link it to cellular processes like vesicle transport, cell migration, and apoptosis regulation. Dysregulation of REPS2 has been implicated in cancer progression, neurological disorders, and cardiovascular diseases, making it a potential therapeutic target. Recombinant human REPS2 protein is typically produced via bacterial or mammalian expression systems for functional studies, structural analysis, and drug discovery. Its modular architecture and role in cross-talk between signaling networks underscore its importance in both basic research and clinical applications. Recent advances in proteomics and CRISPR screening continue to unravel its context-specific interactions and pathophysiological relevance.
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