| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SACM1L |
| Uniprot No | Q9NTJ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-587 aa |
| 活性数据 | MATAAYEQLKLHITPEKFYVEACDDGADDVLTIDRVSTEVTLAVKKDVPPSAVTRPIFGILGTIHLVAGNYLIVITKKIKVGEFFSHVVWKATDFDVLSYKKTMLHLTDIQLQDNKTFLAMLNHVLNVDGFYFSTTYDLTHTLQRLSNTSPEFQEMSLLERADQRFVWNGHLLRELSAQPEVHRFALPVLHGFITMHSCSINGKYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHYNGSKASFVQTRGSIPVFWSQRPNLKYKPLPQISKVANHMDGFQRHFDSQVIIYGKQVIINLINQKGSEKPLEQTFATMVSSLGSGMMRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAGQVVANQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIFKNAWADNANACAKQYAGTGALKTDFTRTGKRTHLGLIMDGWNSMIRYYKNNFSDGFRQDSIDLFLGNYSVDELESHSPLSVPRDWKFLALPIIMVVAFSMCIICLLMAGDTWTETLAYVLFWGVASIGTFFIILYNGKDFVDAPRLVQKEKID |
| 分子量 | 93.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人SACM1L蛋白相关的参考文献(文献标题经整理简化，内容为模拟摘要)：
1. **《SACM1L regulates phosphoinositide metabolism and ER morphology through its lipid transfer activity》**
- 作者：Lees JA, et al.
- 摘要：研究发现SACM1L通过其脂质转移酶结构域调控细胞内膜结构(如内质网)，并参与磷酸肌醇代谢，重组蛋白实验证实其与PI4P(磷脂酰肌醇-4-磷酸)结合能力，影响膜运输与细胞信号通路。
2. **《Structural analysis of SACM1L reveals a conserved role in synaptic vesicle homeostasis》**
- 作者：Zhang Y, et al.
- 摘要：通过重组SACM1L蛋白的晶体结构解析，揭示了其N端PH结构域和C端磷酸酶活性域的构象特征，证明该蛋白在突触囊泡动态平衡和神经元功能中的调控作用。
3. **《SACM1L deficiency disrupts VPS34 complex assembly and promotes autophagy》**
- 作者：Tan X, et al.
- 摘要：利用重组SACM1L蛋白进行体外结合实验，发现其通过与VPS34复合物相互作用调控自噬起始，缺失导致溶酶体功能障碍，提示其在癌症与神经退行性疾病中的潜在作用。
注：以上文献为示例性质，实际文献需通过学术数据库(如PubMed)检索确认。建议使用关键词“SACM1L recombinant protein”“SACM1L phosphoinositide”进一步查找近年研究。
**Background of Recombinant Human SACM1L Protein**
SACM1L (Suppressor of Actin Mutations 1-Like), also known as SAC1. is a conserved phosphatidylinositol phosphatase involved in regulating phosphoinositide metabolism, crucial for maintaining cellular membrane dynamics and signaling. It belongs to the SAC domain-containing protein family, characterized by its ability to hydrolyze phosphatidylinositol 4-phosphate (PI4P) and phosphatidylinositol 3-phosphate (PI3P), modulating lipid gradients essential for organelle identity and vesicular trafficking. SACM1L localizes to endoplasmic reticulum (ER)-Golgi contact sites, endosomes, and the plasma membrane, where it influences Golgi morphology, secretory pathways, and autophagy.
Dysregulation of SACM1L is linked to neurodevelopmental disorders, cancer, and metabolic diseases, with studies suggesting roles in cell proliferation, apoptosis, and neuronal function. Its recombinant form, produced via expression systems like *E. coli* or mammalian cells, enables *in vitro* studies to dissect molecular mechanisms, screen drug candidates, or explore structural interactions. Recombinant SACM1L retains enzymatic activity, facilitating research on phosphoinositide-related pathways and therapeutic targeting. This protein’s versatility makes it a focal point in understanding lipid-mediated cellular regulation and disease pathogenesis.
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