| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VANGL1 |
| Uniprot No | Q8TAA9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-524aa |
| 氨基酸序列 | MDTESTYSGYSYYSSHSKKSHRQGERTRERHKSPRNKDGRGSEKSVTIQPPTGEPLLGND STRTEEVQDDNWGETTTAITGTSEHSISQEDIARISKDMEDSVGLDCKRYLGLTVASFLG LLVFLTPIAFILLPPILWRDELEPCGTICEGLFISMAFKLLILLIGTWALFFRKRRADMP RVFVFRALLLVLIFLFVVSYWLFYGVRILDSRDRNYQGIVQYAVSLVDALLFIHYLAIVL LELRQLQPMFTLQVVRSTDGESRFYSLGHLSIQRAALVVLENYYKDFTIYNPNLLTASKF RAAKHMAGLKVYNVDGPSNNATGQSRAMIAAAARRRDSSHNELYYEEAEHERRVKKRKAR LVVAVEEAFIHIQRLQAEEQQKAPGEVMDPREAAQAIFPSMARALQKYLRITRQQNYHSM ESILQHLAFCITNGMTPKAFLERYLSAGPTLQYDKDRWLSTQWRLVSDEAVTNGLRDGIV FVLKCLDFSLVVNVKKIPFIILSEEFIDPKSHKFVLRLQSETSV |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VANGL1重组蛋白的3篇代表性文献及其摘要概述：
1. **文献名称**：*VANGL1重组蛋白的结构解析及其在平面细胞极性通路中的作用*
**作者**：Devenport D. et al.
**摘要**：该研究通过重组表达纯化VANGL1蛋白，解析其跨膜结构域的三维结构，揭示了其与Prickle蛋白相互作用的分子机制，为平面细胞极性(PCP)通路的信号传递提供结构基础。
2. **文献名称**：*重组VANGL1在结直肠癌细胞迁移中的功能研究*
**作者**：Caldwell G.M. et al.
**摘要**：利用重组VANGL1蛋白体外处理结直肠癌细胞，发现其通过激活Wnt/非经典通路促进细胞迁移和侵袭，提示VANGL1在肿瘤转移中的潜在调控作用。
3. **文献名称**：*VANGL1重组蛋白在神经管发育缺陷模型中的应用*
**作者**：Wnt J. et al.
**摘要**：通过构建VANGL1重组蛋白突变体，研究其在胚胎神经管闭合过程中的功能，发现特定结构域突变导致PCP信号紊乱，从而诱发神经管畸形。
(注：以上文献信息为示例性质，实际文献需通过PubMed或专业数据库检索核实。)
VANGL1 (VANGL planar cell polarity protein 1) is a key component of the planar cell polarity (PCP) pathway, an evolutionarily conserved signaling system critical for tissue polarization and morphogenesis during embryonic development. Encoded by the VANGL1 gene in humans, this transmembrane protein regulates cell orientation, migration, and asymmetric cell division by coordinating signaling events across epithelial and mesenchymal tissues. Structurally, VANGL1 contains four transmembrane domains and a PDZ-binding motif, enabling interactions with other PCP proteins like Frizzled and Dishevelled to propagate polarity signals.
Biologically, VANGL1 is essential for neural tube closure, cochlear hair cell alignment, and renal tubule formation. Dysregulation of VANGL1 is linked to congenital anomalies, including neural tube defects (e.g., spina bifida) and polycystic kidney disease. Additionally, aberrant VANGL1 expression has been implicated in cancers, where it may promote tumor invasion or metastasis through mechanisms involving Wnt/PCP signaling.
Recombinant VANGL1 protein, produced via heterologous expression systems (e.g., HEK293 or E. coli), retains functional domains for in vitro studies. Researchers utilize it to investigate PCP signaling dynamics, screen for therapeutic compounds, or generate antibodies. Its applications extend to disease modeling, particularly in studying developmental disorders and cancer progression. By enabling precise control over protein purity and post-translational modifications, recombinant VANGL1 serves as a vital tool for dissecting its role in development and pathology, offering potential insights for therapeutic targeting.
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