| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3PX3 |
| Uniprot No | Q8WV41 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-574 aa |
| 活性数据 | MALKGRALYD FHSENKEEIS IQQDEDLVIF SETSLDGWLQ GQNSRGETGL FPASYVEIVR SGISTNHADY SSSPAGSPGA QVSLYNSPSV ASPARSGGGS GFLSNQGSFE EDDDDDWDDW DDGCTVVEEP RAGGLGTNGH PPLNLSYPGA YPSQHMAFRP KPPLERQDSL ASAKRGSVVG RNLNRFSCFV RSGVEAFILG DVPMMAKIAE TYSIEMGPRG PQWKANPHPF ACSVEDPTKQ TKFKGIKSYI SYKLTPTHAA SPVYRRYKHF DWLYNRLLHK FTVISVPHLP EKQATGRFEE DFIEKRKRRL ILWMDHMTSH PVLSQYEGFQ HFLSCLDDKQ WKMGKRRAEK DEMVGASFLL TFQIPTEHQD LQDVEDRVDT FKAFSKKMDD SVLQLSTVAS ELVRKHVGGF RKEFQKLGSA FQAISHSFQM DPPFCSEALN SAISHTGRTY EAIGEMFAEQ PKNDLFQMLD TLSLYQGLLS NFPDIIHLQK GAFAKVKESQ RMSDEGRMVQ DEADGIRRRC RVVGFALQAE MNHFHQRREL DFKHMMQNYL RQQILFYQRV GQQLEKTLRM YDNL |
| 分子量 | 65.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SH3PX3蛋白的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"Recombinant Human SH3PX3 Facilitates Clathrin-Mediated Endocytosis through Dynamin Interaction"*
**作者**：Chen L, Wang H, et al. (2016)
**摘要**：该研究报道了重组SH3PX3蛋白在体外与动力素(Dynamin)和网格蛋白(Clathrin)的相互作用，证实其通过SH3结构域招募下游分子，促进囊泡分离，为内吞机制提供了新的调控视角。
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2. **文献名称**：*"Crystal Structure of the SH3-PX Tandem Domains in SH3PX3 Reveals a Unique Lipid-Binding Mechanism"*
**作者**：Kumar S, Yoshida Y, et al. (2019)
**摘要**：作者解析了SH3PX3的SH3-PX双结构域晶体结构，发现PX结构域特异性结合磷脂酰肌醇-3-磷酸(PI3P)，而SH3结构域负责蛋白互作，表明其在膜变形和运输中的双重作用。
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3. **文献名称**：*"SH3PX3 Knockdown Impairs Autophagic Flux: Insights from Recombinant Protein Rescue Experiments"*
**作者**：Rodriguez A, Kim J, et al. (2021)
**摘要**：通过重组SH3PX3蛋白回补实验，研究证明其通过调控自噬体与溶酶体融合维持自噬流，缺失导致神经细胞异常蛋白聚集，提示其与神经退行性疾病的潜在关联。
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**注**：上述文献为示例，实际研究中可能需要根据具体名称查询真实数据库。若SH3PX3相关研究较少，可拓展检索其同源蛋白(如SNX9、SNX33等)或结合领域关键词(如“内吞”“自噬”)。
**Background of Recombinant Human SH3PX3 Protein**
SH3PX3 (SH3 domain-containing protein 3), also known as SNX9 (Sorting Nexin 9), is a multi-domain protein involved in membrane trafficking, endocytosis, and cytoskeletal dynamics. It belongs to the sorting nexin family, characterized by a Phox homology (PX) domain that binds phosphoinositides, directing membrane association. SH3PX3 additionally contains an SH3 domain for interacting with proline-rich motifs in partner proteins (e.g., dynamin, clathrin), and a BAR domain that senses or induces membrane curvature during vesicle formation.
This protein plays a critical role in clathrin-mediated endocytosis, where it coordinates cargo sorting, membrane remodeling, and actin polymerization. It acts as a scaffold, linking endocytic machinery to actin regulators like N-WASP, facilitating vesicle scission and intracellular transport. Dysregulation of SH3PX3 is implicated in cancer metastasis, neurological disorders, and viral entry mechanisms, reflecting its importance in cellular homeostasis.
Recombinant human SH3PX3 protein is produced in vitro using expression systems (e.g., *E. coli* or mammalian cells*) for functional studies. It serves as a tool to explore endocytosis pathways, protein-protein interactions, and therapeutic targeting in diseases linked to trafficking defects. Its recombinant form enables structural analysis, antibody development, and high-throughput drug screening, advancing both basic research and translational applications.
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