| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC22A8 |
| Uniprot No | Q8TCC7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-542 aa |
| 活性数据 | MTFSEILDRVGSMGHFQFLHVAILGLPILNMANHNLLQIFTAATPVHHCRPPHNASTGPWVLPMGPNGKPERCLRFVHPPNASLPNDTQRAMEPCLDGWVYNSTKDSIVTEWDLVCNSNKLKEMAQSIFMAGILIGGLVLGDLSDRFGRRPILTCSYLLLAASGSGAAFSPTFPIYMVFRFLCGFGISGITLSTVILNVEWVPTRMRAIMSTALGYCYTFGQFILPGLAYAIPQWRWLQLTVSIPFFVFFLSSWWTPESIRWLVLSGKSSKALKILRRVAVFNGKKEEGERLSLEELKLNLQKEISLAKAKYTASDLFRIPMLRRMTFCLSLAWFATGFAYYSLAMGVEEFGVNLYILQIIFGGVDVPAKFITILSLSYLGRHTTQAAALLLAGGAILALTFVPLDLQTVRTVLAVFGKGCLSSSFSCLFLYTSELYPTVIRQTGMGVSNLWTRVGSMVSPLVKITGEVQPFIPNIIYGITALLGGSAALFLPETLNQPLPETIEDLENWSLRAKKPKQEPEVEKASQRIPLQPHGPGLGSS |
| 分子量 | 86.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人SLC22A8(OAT3)蛋白的研究文献摘要：
1. **文献名称**: *"Role of the human kidney transporter OAT3 (SLC22A8) in the renal clearance of mycophenolic acid"*
**作者**: VanWert AL, et al.
**摘要**: 该研究揭示SLC22A8/OAT3在肾脏中对免疫抑制剂霉酚酸(MPA)的主动分泌起关键作用，解释了其临床药代动力学特性。
2. **文献名称**: *"Downregulation of Organic Anion Transporter 3 (SLC22A8) in Renal Ischemia-Reperfusion Injury"*
**作者**: Schneider R, et al.
**摘要**: 发现肾缺血再灌注损伤可导致SLC22A8表达显著下调，可能通过抑制有机阴离子排泄加重肾脏损伤。
3. **文献名称**: *"Interaction of Uremic Toxins with Renal OAT (SLC22A) Transporters"*
**作者**: Mutsaers HAM, et al.
**摘要**: 证明SLC22A8/OAT3在慢性肾病中清除硫酸吲哚酚等尿毒症毒素的功能受损，与毒素蓄积相关的病理机制密切相关。
4. **文献名称**: *"Structural Determinants of Substrate Specificity in the Human OAT3 (SLC22A8) Transporter"*
**作者**: Zhou F, et al.
**摘要**: 通过定点突变分析发现SLC22A8的跨膜结构域中特定氨基酸残基对底物识别和转运活性具有决定性作用。
Recombinant human SLC22A8 protein, also known as organic anion transporter 3 (OAT3), is a member of the solute carrier family 22 (SLC22) responsible for mediating the transport of organic anions across cellular membranes. Primarily expressed in the kidneys, liver, and brain, OAT3 plays a critical role in the elimination of endogenous metabolites (e.g., prostaglandins, uric acid) and xenobiotics, including drugs (e.g., antibiotics, diuretics) and environmental toxins. It functions as a basolateral membrane transporter in renal proximal tubules, facilitating the uptake of organic anions from blood into cells for subsequent excretion into urine.
The recombinant form of SLC22A8 is produced using engineered cell systems (e.g., HEK293 or insect cells) to enable functional and structural studies. Research on recombinant OAT3 aids in understanding drug-drug interactions, pharmacokinetics, and toxicity mechanisms, as many therapeutics are OAT3 substrates or inhibitors. Structural analyses suggest 12 transmembrane domains with intracellular N- and C-termini, and key residues involved in substrate recognition have been identified. Genetic polymorphisms in SLC22A8 may contribute to interindividual variability in drug responses and disease susceptibility. Its role in transporting signaling molecules (e.g., steroid hormones) also links it to metabolic and endocrine regulation, expanding its biomedical relevance beyond detoxification pathways.
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