| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC29A1 |
| Uniprot No | Q99808 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-456 aa |
| 活性数据 | MTTSHQPQDRYKAVWLIFFMLGLGTLLPWNFFMTATQYFTNRLDMSQNVSLVTAELSKDAQASAAPAAPLPERNSLSAIFNNVMTLCAMLPLLLFTYLNSFLHQRIPQSVRILGSLVAILLVFLITAILVKVQLDALPFFVITMIKIVLINSFGAILQGSLFGLAGLLPASYTAPIMSGQGLAGFFASVAMICAIASGSELSESAFGYFITACAVIILTIICYLGLPRLEFYRYYQQLKLEGPGEQETKLDLISKGEEPRAGKEESGVSVSNSQPTNESHSIKAILKNISVLAFSVCFIFTITIGMFPAVTVEVKSSIAGSSTWERYFIPVSCFLTFNIFDWLGRSLTAVFMWPGKDSRWLPSLVLARLVFVPLLLLCNIKPRRYLTVVFEHDAWFIFFMAAFAFSNGYLASLCMCFGPKKVKPAEAETAGAIMAFFLCLGLALGAVFSFLFRAIV |
| 分子量 | 76.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SLC29A1(hENT1)蛋白的3篇代表性文献信息：
1. **文献名称**：**Crystal structure of a human ENT nucleoside transporter reveals structural basis for substrate recognition**
**作者**：Lee, J.Y., et al.
**摘要**：该研究解析了人源SLC29A1(hENT1)的晶体结构，揭示了其底物(如腺苷和化疗药物吉西他滨)的结合位点及跨膜转运机制，为设计靶向该蛋白的药物提供了结构基础。
2. **文献名称**：**Role of human equilibrative nucleoside transporter 1 (hENT1) in the cellular uptake of nucleoside analog drugs and progression of colorectal cancer**
**作者**：Zhang, J., et al.
**摘要**：研究通过细胞和动物模型证明，SLC29A1表达水平影响结直肠癌细胞对核苷类似物(如吉西他滨)的摄取和化疗敏感性，同时其表达下调可能导致癌症耐药性。
3. **文献名称**：**Regulation of human equilibrative nucleoside transporter 1 (hENT1) by hypoxia-inducible factors in pancreatic cancer**
**作者**：Wong, N., et al.
**摘要**：发现胰腺癌细胞中低氧微环境通过HIF-1α调控SLC29A1表达，降低其对吉西他滨的摄取和疗效，提示靶向HIF信号通路或可改善化疗效果。
**备注**：实际应用中建议通过PubMed或Web of Science平台用关键词“SLC29A1”“hENT1”“recombinant”等检索近年高质量论文，优先选择《Nature》《Cell》子刊或领域内权威期刊的研究。
Recombinant human SLC29A1 protein, also known as equilibrative nucleoside transporter 1 (ENT1), belongs to the solute carrier 29 (SLC29) family. It is a transmembrane protein primarily involved in the cellular uptake and efflux of nucleosides, nucleobases, and nucleoside analog drugs. SLC29A1 facilitates bidirectional transport across cell membranes through an energy-independent mechanism, relying on concentration gradients. Structurally, it consists of 11 predicted transmembrane domains, with intracellular N- and C-termini, and plays a critical role in maintaining nucleoside homeostasis, which is essential for DNA/RNA synthesis, cell signaling, and energy metabolism.
This protein is widely expressed in various tissues, including the liver, kidney, brain, and heart, and is particularly significant in pharmacology due to its involvement in the cellular uptake of chemotherapeutic agents (e.g., gemcitabine) and antiviral drugs (e.g., zalcitabine). Dysregulation or mutations in SLC29A1 have been linked to drug resistance in cancer therapy and certain genetic disorders. Recombinant SLC29A1 is produced using heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies, drug interaction assays, and structural characterization. Its study aids in understanding transporter-mediated pharmacokinetics and developing strategies to improve therapeutic efficacy by modulating nucleoside analog bioavailability.
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