| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC35B2 |
| Uniprot No | O00221 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-500 aa |
| 活性数据 | MNQRRSESRP GNHRLQAYAE PGKGDSGGAG PLSGSARRGR GGGGAIRVRR PCWSGGAGRG GGPAWAVRLP TVTAGWTWPA LRTLSSLRAG PSEPHSPGRR PPRAGRPLCQ ADPQPGKR RSLEPDPAQT GPRPARGM SEARKGPDEA EESQYDSGIE SLRSLRSLPE STSAPASGPS DGSPQPCTHP PGPVKEPQEK EDADGERADS TYGSSSLTYT LSLLGGPEAE DPAPRLPLPH VGALSPQQLE ALTYISEDGD TLVHLAVIHE APAVLLCCLA LLPQEVLDIQ NNLYQTALHL AVHLDQPGAV RALVLKGASR ALQDRHGDTA LHVACQRQHL ACARCLLEGR PEPGRGTSHS LDLQLQNWQG LACLHIATLQ KNQPLMELLL RNGADIDVQE GTSGKTALHL AVETQERGLV QFLLQAGAQV DARMLNGCTP LHLGRGLM GISSTLCKAG ADSLLRNVED ETPQDLTEES LVLLPFDDLK ISGKLLLCTD |
| 分子量 | 52.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设的3-4条关于重组人SLC35B2蛋白的参考文献示例(仅作参考，需根据实际文献调整)：
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1. **文献名称**: *Functional characterization of recombinant human SLC35B2 as a nucleotide sugar transporter in vitro*
**作者**: Smith A, et al. (2015)
**摘要**: 该研究通过在大肠杆菌中表达并纯化重组人SLC35B2蛋白，验证其作为UDP-木糖/UDP-N-乙酰葡萄糖胺转运体的功能，并证明其在内质网中参与蛋白糖基化修饰的作用。
2. **文献名称**: *Structural insights into the substrate specificity of SLC35B2 through cryo-EM analysis*
**作者**: Zhang L, et al. (2020)
**摘要**: 利用冷冻电镜解析了重组SLC35B2蛋白的三维结构，揭示了其底物结合口袋的关键氨基酸残基，解释了其对UDP-木糖的选择性运输机制。
3. **文献名称**: *SLC35B2 overexpression promotes tumor metastasis via altered glycosylation in breast cancer models*
**作者**: Lee H, et al. (2021)
**摘要**: 通过体外重组SLC35B2蛋白功能实验，发现其过表达改变癌细胞表面糖基化模式，增强细胞迁移能力，提示其作为癌症治疗潜在靶点的可能性。
4. **文献名称**: *A high-throughput assay for screening SLC35B2 inhibitors using fluorescence-based substrate uptake*
**作者**: Wang Y, et al. (2022)
**摘要**: 开发了一种基于重组SLC35B2蛋白的荧光标记底物转运高通量筛选体系，用于发现特异性抑制剂，为靶向糖代谢的药物研发提供工具。
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**提示**：以上文献为模拟示例。实际研究中建议通过PubMed、Google Scholar等平台使用关键词“SLC35B2 recombinant”“SLC35B2 transporter”或结合具体研究领域(如癌症、糖基化)检索真实文献。
Recombinant human SLC35B2 protein is a key transporter belonging to the solute carrier family 35 (SLC35), which facilitates the movement of nucleotide sugars across intracellular membranes. SLC35B2. also known as UXT1 or PAPST1. localizes primarily to the endoplasmic reticulum and Golgi apparatus, where it plays a critical role in glycosylation processes. Specifically, it transports UDP-xylose and UDP-glucuronic acid, essential substrates for synthesizing proteoglycans and glycoconjugates. These molecules are vital for cell-cell communication, extracellular matrix structure, and protein functionality.
Structurally, SLC35B2 contains 10 transmembrane domains, a hallmark of nucleotide sugar transporters. Recombinant versions are produced using expression systems (e.g., mammalian or bacterial cells) to study its biochemical properties, substrate specificity, and interactions with glycosylation enzymes. Dysregulation of SLC35B2 has been linked to glycosylation disorders, cancer progression, and inflammatory diseases, highlighting its biomedical relevance. Research on recombinant SLC35B2 aids in elucidating mechanisms of congenital disorders of glycosylation (CDGs) and exploring therapeutic strategies targeting glycosylation pathways. Its application extends to drug development, biomarker discovery, and understanding metabolic reprogramming in tumors.
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