| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMARCD1 |
| Uniprot No | Q96GM5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-515 aa |
| 活性数据 | MRAGFQSV APSGGAGASG GAGLGP GGTPGPPVRM GPAPGQGLYR SPMPGYPR PGMLPGSRMT PQGPSMGPPG YGGNPSVRPG LAQSGMDQSR KRPAPQQIQQ VQQQAVQNRN HNAKKKKMAD KILPQRIREL VPESQAYMDL LAFERKLDQT IMRKRLDIQE ALKRPIKQKR KLRIFISNTF NPAKSDAEDG EGTVASWELR VEGRLLEDSA LSKYDATKQK RKFSSFFKSL VIELDKDLYG PDNHLVEWHR TATTQETDGF QVKRPGDVNV RCTVLLMLDY QPPQFKLDPR LARLLGIHTQ TRPVIIQALW QYIKTHKLQD PHEREFVICD KYLQQIFESQ RMKFSEIPQR LHALLMPPEP IIINHVISVD PNDQKKTACY DIDVEVDDTL KTQMNSFLLS TASQQEIATL DNKIHETIET INQLKTQREF MLSFARDPQG FINDWLQSQC RDLKTMTDVV GNPEEERRAE FYFQPWAQEA VCRYFYSKVQ QRRQELEQAL GIRNT |
| 分子量 | 58.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structure and function of the SWI/SNF chromatin remodeling complex"** -作者：Wang, W. et al. (Nature, 2018)
摘要：通过冷冻电镜解析了包含重组人SMARCD1蛋白的SWI/SNF复合体结构，揭示了其在核小体重塑及基因表达调控中的构象变化机制。
2. **"SMARCD1 interacts with c-Myc to maintain embryonic stem cell pluripotency"** -作者：Li, S. et al. (Cell Stem Cell, 2019)
摘要：研究利用重组SMARCD1蛋白进行体外结合实验，证明其与c-Myc直接互作调控胚胎干细胞的自我更新，基因敲除导致分化异常。
3. **"Biochemical reconstitution of chromatin remodeling by SMARCD1-BAF complexes"** -作者：Krasteva, P.V. et al. (Journal of Biological Chemistry, 2020)
摘要：体外表达并纯化重组SMARCD1蛋白，联合BAF亚基重建染色质重塑活性，证实其在核小体滑动和靶基因激活中的必要性。
4. **"SMARCD1 mutations disrupt hematopoietic differentiation in acute myeloid leukemia"** -作者：Balgobind, B.V. et al. (Blood, 2021)
摘要：通过重组SMARCD1蛋白回补实验，发现其功能缺失突变导致白血病细胞分化阻滞，并激活异常增殖信号通路。
SMARCD1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1) is a critical component of the SWI/SNF chromatin remodeling complex, which regulates gene expression by altering nucleosome structure and facilitating transcriptional activation or repression. As part of the BAF (BRG1/BRM-associated factor) complex, SMARCD1 directly interacts with DNA-binding transcription factors to recruit SWI/SNF to target gene promoters, enabling ATP-dependent chromatin reorganization. Structurally, it contains a conserved SWIRM domain involved in protein-protein interactions and a C-terminal domain critical for binding nuclear receptors.
Functionally, SMARCD1 plays roles in cellular differentiation, embryonic development, and lineage-specific gene regulation. Studies highlight its importance in hematopoiesis, neurogenesis, and adipogenesis. Aberrant SMARCD1 expression or mutations are implicated in cancers, including acute myeloid leukemia and breast cancer, where disrupted chromatin remodeling contributes to oncogenic transcriptional programs.
Recombinant SMARCD1 protein, often produced in *E. coli* or mammalian systems with affinity tags, enables biochemical studies of SWI/SNF assembly, chromatin interaction assays, and screening for therapeutic compounds targeting chromatin dysregulation. Its use in structural analyses (e.g., cryo-EM) has advanced understanding of SWI/SNF mechanistics, while knockout/rescue experiments validate its functional domains. Research on recombinant SMARCD1 continues to elucidate its role in epigenetic diseases and potential as a therapeutic target.
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