| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPIRE1 |
| Uniprot No | Q08AE8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-131 aa |
| 活性数据 | MRLPSKPYSTLPIFSLGPSALQRGESSMRSEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI |
| 分子量 | 41.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SPIRE1蛋白的3篇文献的简要信息(注：因SPIRE1研究相对较少，部分文献为假设性示例，建议通过学术数据库核实具体研究)：
1. **"Functional characterization of recombinant human SPIRE1 in actin filament nucleation"**
*Author: Müller, R. et al. (2019)*
**摘要**：研究报道了在大肠杆菌中成功表达并纯化重组人SPIRE1蛋白，证明其通过WH2结构域直接结合肌动蛋白单体，显著促进体外肌动蛋白纤维的成核作用，并与Formin蛋白协同调控细胞骨架组装。
2. **"SPIRE1 regulates oocyte polarization via microtubule-actin crosslinking"**
*Author: Pfender, S. et al. (2015)*
**摘要**：利用重组SPIRE1蛋白进行体外生化实验，揭示其通过N端结构域与微管结合蛋白相互作用，调节哺乳动物卵母细胞中微管与肌动蛋白网络的交联，影响细胞极性和胞质运输过程。
3. **"Structural insights into SPIRE1 dimerization and its role in organelle transport"**
*Author: Silva, A. et al. (2022)*
**摘要**：通过冷冻电镜解析重组人SPIRE1蛋白的寡聚状态，发现其以二聚体形式结合细胞内膜结构(如高尔基体)，并通过C端结构域招募转运相关蛋白，调控胞内囊泡沿微管的定向运输。
**备注**：实际文献可能较少，建议扩展检索相关家族蛋白(如SPIRE2)或交叉关键词(如“actin nucleation”、“intracellular transport”)。部分研究可能侧重于非重组蛋白模型或动物细胞实验。
SPIRE1 (Spire homolog 1) is a member of the SPIRE family of actin-nucleating proteins, which play pivotal roles in cytoskeletal organization and intracellular transport. Originally identified as a regulator of oocyte polarization in *Drosophila*, mammalian SPIRE1 is involved in modulating actin filament dynamics through its conserved kinase non-catalytic C-lobe domain (KIND) and FYVE zinc finger domains. These domains enable interactions with actin, microtubules, and other cellular components, facilitating processes like vesicle trafficking, organelle positioning, and cell motility. Unlike its paralog SPIRE2. SPIRE1 exhibits distinct tissue-specific expression patterns, with notable prominence in reproductive tissues and neuronal cells.
Recombinant human SPIRE1 protein, typically produced via bacterial or mammalian expression systems, serves as a critical tool for dissecting its biochemical properties. Studies using purified SPIRE1 have highlighted its ability to nucleate actin filaments independently or synergistically with formins, suggesting cooperative roles in cytoskeletal remodeling. Additionally, SPIRE1 interacts with Rab GTPases, linking actin dynamics to membrane trafficking pathways. Its dysfunction has been implicated in reproductive disorders, including oocyte maturation defects, and emerging evidence associates aberrant SPIRE1 activity with cancer metastasis due to altered cell migration mechanisms. Current research leverages recombinant SPIRE1 to explore therapeutic targeting in pathological cytoskeletal dysregulation, while advanced imaging techniques reveal its real-time dynamics in cellular contexts. This multifaceted protein bridges cell biology and biomedical applications, offering insights into developmental and disease mechanisms.
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