| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SREBF2 |
| Uniprot No | Q12772 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 801-900 aa |
| 活性数据 | QAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSVGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFT |
| 分子量 | 36.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SREBF2蛋白的3篇代表性文献的简要整理：
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1. **文献名称**: "SREBPs: Activators of the Complete Program of Cholesterol and Fatty Acid Synthesis in the Liver"
**作者**: Horton, J.D. et al.
**摘要**: 该研究阐明了SREBP家族(包括SREBF2)作为调控胆固醇和脂肪酸合成的主要转录因子，揭示了其在肝脏中通过结合靶基因启动子的固醇调控元件(SRE)来激活LDL受体、HMG-CoA还原酶等胆固醇合成相关基因的表达机制。
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2. **文献名称**: "Intramembrane Proteolysis of SREBPs by SCAP Controls Lipogenesis"
**作者**: Goldstein, J.L., Brown, M.S.
**摘要**: 研究发现了SREBF2的激活依赖SCAP蛋白介导的内质网到高尔基体的转运过程，揭示了细胞通过感知胆固醇水平调节SREBF2蛋白水解切割的机制，从而调控脂质代谢稳态。
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3. **文献名称**: "SREBP2 Contributes to Glioblastoma Growth by Enhancing Sterol Synthesis and EGFR Signaling"
**作者**: Guo, D. et al.
**摘要**: 该文献报道SREBF2在胶质母细胞瘤中异常高表达，通过促进胆固醇合成和增强EGFR信号通路驱动肿瘤生长，靶向抑制SREBF2可抑制肿瘤进展，揭示了其在癌症代谢中的关键作用。
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这些文献覆盖了SREBF2的核心调控机制及其在疾病中的功能研究。如需具体文章引用格式或更多文献，可进一步补充。
Sterol Regulatory Element-Binding Transcription Factor 2 (SREBF2), also known as SREBP2. is a key regulatory protein involved in cholesterol homeostasis. It belongs to the SREBP family of transcription factors, which are essential for lipid metabolism regulation. SREBF2 specifically controls genes encoding enzymes in the cholesterol biosynthesis pathway, such as HMG-CoA reductase, and LDL receptor expression. Synthesized as an inactive precursor bound to the endoplasmic reticulum membrane, SREBF2 undergoes proteolytic activation when cellular cholesterol levels are low. The cleaved mature form translocates to the nucleus, binds sterol regulatory elements (SREs), and activates target gene transcription.
Recombinant human SREBF2 protein is widely used to study cholesterol regulation mechanisms, drug development (e.g., statins), and diseases linked to lipid dysregulation, including atherosclerosis and metabolic disorders. Produced via heterologous expression systems (e.g., E. coli, mammalian cells), it enables structural and functional analyses, such as DNA-binding assays, protein interaction studies, and pathway validation. Its application enhances understanding of how SREBF2 mutations or dysfunctions contribute to pathologies, offering insights for therapeutic strategies targeting cholesterol metabolism. Researchers also utilize recombinant SREBF2 to screen compounds modulating lipid biosynthesis, aiding in the discovery of novel lipid-lowering agents.
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