| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GAL6 |
| Uniprot No | Q01532 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483aa |
| 氨基酸序列 | MLPTSVSRSLYLKTFRSHLLRAPQIVLKRMSSSIDISKINSWNKEFQSDLTHQLATTVLKNYNADDALLNKTRLQKQDNRVFNTVVSTDSTPVTNQKSSGRCWLFAATNQLRLNVLSELNLKEFELSQAYLFFYDKLEKANYFLDQIVSSADQDIDSRLVQYLLAAPTEDGGQYSMFLNLVKKYGLIPKDLYGDLPYSTTASRKWNSLLTTKLREFAETLRTALKERSADDSIIVTLREQMQREIFRLMSLFMDIPPVQPNEQFTWEYVDKDKKIHTIKSTPLEFASKYAKLDPSTPVSLINDPRHPYGKLIKIDRLGNVLGGDAVIYLNVDNETLSKLVVKRLQNNKAVFFGSHTPKFMDKKTGVMDIELWNYPAIGYNLPQQKASRIRYHESLMTHAMLITGCHVDETSKLPLRYRVENSWGKDSGKDGLYVMTQKYFEEYCFQIVVDINELPKELASKFTSGKEEPIVLPIWDPMGALAK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GAL6重组蛋白的模拟参考文献示例(注：内容为虚构，仅供参考)：
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1. **文献名称**：*"High-yield Expression and Functional Analysis of Recombinant GAL6 in Insect Cells"*
**作者**：Zhang, Y. et al.
**摘要**：本研究利用杆状病毒-昆虫细胞系统高效表达GAL6重组蛋白，通过亲和层析纯化获得高纯度蛋白，并验证其与半乳糖衍生物的特异性结合能力，为后续结构功能研究奠定基础。
2. **文献名称**：*"GAL6 Recombinant Protein Attenuates Autoimmune Response in Murine Models"*
**作者**：Smith, J.L. et al.
**摘要**：通过大肠杆菌表达重组GAL6蛋白，发现其在小鼠模型中显著抑制T细胞过度活化，提示GAL6可能通过调控免疫检查点通路治疗自身免疫疾病。
3. **文献名称**：*"Crystallographic Characterization of GAL6: Insights into Substrate Recognition Mechanisms"*
**作者**：Tanaka, R. et al.
**摘要**：解析了重组GAL6的X射线晶体结构(分辨率2.1Å)，揭示了其底物结合口袋的关键氨基酸残基，为设计靶向抑制剂提供结构依据。
4. **文献名称**：*"Optimizing GAL6 Production in E. coli via Codon Usage and Induction Strategy Modifications"*
**作者**：Kumar, S. et al.
**摘要**：通过密码子优化及诱导条件调整，将GAL6在大肠杆菌中的可溶性表达量提升至80%，解决了包涵体形成问题，适用于规模化制备。
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建议通过PubMed、Google Scholar或Web of Science等平台，以关键词**"recombinant GAL6 protein"**或**"GAL6 expression"**检索真实文献。
**Background of GAL6 Recombinant Protein**
GAL6. a member of the galactose-regulated (GAL) protein family, is primarily associated with carbohydrate metabolism and signaling pathways, particularly in yeast and other eukaryotic organisms. Originally identified in *Saccharomyces cerevisiae*, GAL-family proteins are crucial for the cellular response to galactose, regulating genes involved in galactose utilization. GAL6. though less characterized than its homologs (e.g., GAL4 or GAL80), is hypothesized to play a role in modulating transcriptional regulation or metabolic adaptation under specific nutrient conditions.
Recombinant GAL6 protein is engineered using genetic cloning techniques, where the *GAL6* gene is inserted into expression vectors (e.g., bacterial or yeast systems) to enable large-scale production. This allows researchers to study its structure, function, and interactions in vitro. The recombinant protein is often purified via affinity tags (e.g., His-tag) and validated through assays like SDS-PAGE or Western blotting.
Studies on GAL6 recombinant protein aim to elucidate its precise biological role, particularly its potential involvement in sugar-sensing pathways, stress responses, or cross-talk with other regulatory networks. Its applications extend to industrial biotechnology, such as optimizing microbial fermentation processes, or in biomedical research to explore metabolic disorders linked to carbohydrate dysregulation.
Despite limited direct literature on GAL6. its homology to well-studied GAL proteins suggests it may serve as a regulatory component or adaptor in galactose-mediated signaling. Further research is needed to clarify its mechanisms and expand its utility in synthetic biology or therapeutic contexts.
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