| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THUMPD2 |
| Uniprot No | Q9BTF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-503 aa |
| 活性数据 | MSEARGEPGS GPEAGARFFC TAGRGLEPFV MREVRARLAA TQVEYISGKV FFTTCSDLNM LKKLKSAERL FLLIKKQFPL IISSVSKGKI FNEMQRLINE DPGSWLNAIS IWKNLLELDA KKEKLSQRDD NQLKRKVGEN EIIAKKLKIE QMQKIEENRD CQLEKQIKEE TLEQRDFTTK SEKFQEEEFQ NDIEKAIDTH NQNDLTFRVS CRCSGTIGKA FTAQEVGKVI GIAIMKHFGW KADLRNPQLE IFIHLNDIYS VVGIPVFRVS LASRAYIKTA GLRSTIAWAM ASLADIKAGA FVLDPMCGLG TILLEAAKEW PDVYYVGADV SDSQLLGTWD NLKAAGLEDK IELLKISVIE LPLPSESVDI IISDIPFGKK FKLGKDIKSI LQEMERVLHV GGTIVLLLSE DHHRRLTDCK ESNIPFNSKD SHTDEPGIKK CLNPEEKTGA FKTASTSFEA SNHKFLDRMS PFGSLVPVEC YKVSLGKTDA FICKYKKSHS SGL |
| 分子量 | 56.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人THUMPD2蛋白的假设参考文献示例(供参考，具体文献需通过学术数据库验证)：
1. **文献名称**：Structural and Functional Analysis of Human THUMPD2 as an RNA Methyltransferase
**作者**：Zhang Y. et al.
**摘要**：研究解析了重组表达的THUMPD2蛋白的晶体结构，揭示其THUMP结构域在RNA结合和甲基转移酶活性中的作用，并提出其可能参与rRNA修饰的分子机制。
2. **文献名称**：Recombinant THUMPD2 Interaction with Small RNAs in Human Cells
**作者**：Li X. et al.
**摘要**：通过体外重组表达和RNA pull-down实验，发现THUMPD2优先结合特定小RNA分子(如tRNA片段)，表明其在RNA代谢与细胞应激反应中的潜在功能。
3. **文献名称**：Expression and Purification of Active THUMPD2 Protein in E. coli
**作者**：Wang H. et al.
**摘要**：描述了人源THUMPD2在大肠杆菌中的高效重组表达及纯化策略，并通过酶活实验验证其甲基转移酶活性，为功能研究提供工具。
4. **文献名称**：THUMPD2 Deficiency Alters Ribosomal RNA Processing in Mammalian Cells
**作者**：Chen L. et al.
**摘要**：利用重组蛋白互补实验，证明THUMPD2缺失导致核糖体RNA加工异常，提示其在RNA质量控制中的关键作用。
**注意**：以上文献为示例性概括，实际研究可能存在差异，建议通过**PubMed/Google Scholar**检索关键词“THUMPD2”或“THUMP domain-containing protein 2”获取真实文献。
Here’s a concise description of recombinant human THUMPD2 protein:
Recombinant human THUMPD2 (THU domain-containing protein 2) is a genetically engineered protein belonging to the THUMP family, characterized by a conserved THUMP (THO complex and RNA recognition motif) domain at its N-terminus. This domain is implicated in RNA binding and metabolism, suggesting potential roles in post-transcriptional regulation. The C-terminal region contains a putative S-adenosylmethionine (SAM)-binding methyltransferase domain, though its enzymatic activity remains under investigation. THUMPD2 is broadly expressed in human tissues, with higher levels observed in proliferating cells, and has been linked to cancer progression. Studies associate its overexpression with enhanced tumor cell survival, migration, and chemoresistance in malignancies such as hepatocellular carcinoma and breast cancer. Recombinant THUMPD2 is typically produced in bacterial or mammalian expression systems for functional studies. It serves as a tool to investigate RNA-protein interactions, methyltransferase activity, and its oncogenic mechanisms. Recent interest focuses on exploring THUMPD2 as a potential diagnostic biomarker or therapeutic target, particularly in RNA-modification-related pathologies. However, its precise biological functions and substrates require further elucidation through structural and interactome analyses.
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