关键信息
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基因名
fabD
- 应用
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别名
fabD；MT；Malonyl-CoA-acyl carrier Protein transacylase. mitochondrial
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q5HGK3
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表达区间
1-308aa
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氨基酸序列
MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDILETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVLSFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANINCPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFEWRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPGKVLSGLIKKINRDVKLTSIQTLEDVKGWNEND
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分子量
35.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
FabD is a key enzyme involved in fatty acid biosynthesis, specifically in the acyl-ACP (acyl carrier protein) synthetase family, which plays a crucial role in the metabolic pathways of various organisms, including bacteria and higher plants. As a member of this enzyme family, FabD catalyzes the conversion of malonyl-CoA to malonyl-ACP, a critical step in the synthesis of fatty acids. The study of FabD is particularly important due to its implications in microbial growth and survival, making it a target for the development of antibiotics and other antimicrobial agents. Furthermore, understanding FabD's structure and function can offer insights into the regulation of fatty acid metabolism and its impact on cellular processes, such as membrane fluidity, energy storage, and signaling pathways. The recombinant expression of FabD allows researchers to characterize its enzymatic properties, elucidate its mechanism of action, and explore its potential applications in biotechnology, including biofuel production and metabolic engineering. Overall, the investigation of FabD and its recombinant protein can provide valuable information for both fundamental biology and applied sciences, supporting efforts to manipulate fatty acid biosynthesis for various industrial and pharmaceutical purposes.
