关键信息
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基因名
SPHK1
- 应用
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别名
SPHK1；SK1；SPHK；SPK；Sphingosine kinase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NYA1
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表达区间
1-384aa
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氨基酸序列
MDPAGGPRGV LPRPCRVLVL LNPRGGKGKA LQLFRSHVQP LLAEAEISFT LMLTERRNHA RELVRSEELG RWDALVVMSG DGLMHEVVNG LMERPDWETA IQKPLCSLPA GSGNALAASL NHYAGYEQVT NEDLLTNCTL LLCRRLLSPM NLLSLHTASG LRLFSVLSLA WGFIADVDLE SEKYRRLGEM RFTLGTFLRL AALRTYRGRL AYLPVGRVGS KTPASPVVVQ QGPVDAHLVP LEEPVPSHWT VVPDEDFVLV LALLHSHLGS EMFAAPMGRC AAGVMHLFYV RAGVSRAMLL RLFLAMEKGR HMEYECPYLV YVPVVAFRLE PKDGKGVFAV DGELMVSEAV QGQVHPNYFW MVSGCVEPPP SWKPQQMPPP EEPL
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SPHK1 (Sphingosine Kinase 1) is an important enzyme involved in sphingolipid metabolism, converting sphingosine to sphingosine-1-phosphate (S1P), a bioactive lipid mediator that plays critical roles in various cellular processes, including cell proliferation, survival, migration, and apoptosis. Dysregulation of SPHK1 has been implicated in various diseases, such as cancer, cardiovascular disorders, and neurodegenerative conditions. Given its significant role in cell signaling and disease progression, SPHK1 has emerged as a potential therapeutic target. The production and characterization of recombinant SPHK1 proteins have become crucial for elucidating its structure-function relationship and for developing inhibitors or modulators that can interfere with its activity. Recent advances in recombinant DNA technology and protein expression systems have facilitated the high-yield production of SPHK1, allowing for in-depth biochemical studies and structural analyses. Understanding the functional dynamics of SPHK1 through recombinant techniques not only helps in deciphering its cellular mechanisms but also aids in the rational design of novel therapeutic strategies aimed at diseases linked to sphingolipid signaling pathways.
