关键信息
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基因名
COG3
- 应用
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别名
COG complex subunit 3; Cog3; COG3_HUMAN; Component of golgi transport complex 3; Component of oligomeric Golgi complex 3; Conserved oligomeric Golgi complex subunit 3; OTTHUMP00000018352; p94; SEC34; SEC34 S
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96JB2
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表达区间
1-828aa
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氨基酸序列
MAEAALLLLPEAAAERDAREKLALWDRRPDTTAPLTDRQTDSVLELKAAAENLPVPAELPIEDLCSLTSQSLPIELTSVVPESTEDILLKGFTSLGMEEERIETAQQFFSWFAKLQTQMDQDEGTKYRQMRDYLSGFQEQCDAILNDVNSALQHLESLQKQYLFVSNKTGTLHEACEQLLKEQSELVDLAENIQQKLSYFNELETINTKLNSPTLSVNSDGFIPMLAKLDDCITYISSHPNFKDYPIYLLKFKQCLSKALHLMKTYTVNTLQTLTSQLLKRDPSSVPNADNAFTLFYVKFRAAAPKVRTLIEQIELRSEKIPEYQQLLNDIHQCYLDQRELLLGPSIACTVAELTSQNNRDHCALVRSGCAFMVHVCQDEHQLYNEFFTKPTSKLDELLEKLCVSLYDVFRPLIIHVIHLETLSELCGILKNEVLEDHVQNNAEQLGAFAAGVKQMLEDVQERLVYRTHIYIQTDITGYKPAPGDLAYPDKLVMMEQIAQSLKDEQKKVPSEASFSDVHLEEGESNSLTKSGSTESLNPRPQTTISPADLHGMWYPTVRRTLVCLSKLYRCIDRAVFQGLSQEALSACIQSLLGASESISKNKTQIDGQLFLIKHLLILREQIAPFHTEFTIKEISLDLKKTRDAAFKILNPMTVPRFFRLNSNNALIEFLLEGTPEIREHYLDSKKDVDRHLKSACEQFIQQQTKLFVEQLEEFMTKVSALKTMASQGGPKYTLSQQPWAQPAKVSDLAATAYKTIKTKLPVTLRSMSLYLSNKDTEFILFKPVRNNIQQVFQKFHALLKEEFSPEDIQIIACPSMEQLSLLLSVSK
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分子量
120.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
COG3 is a key component of the conserved oligomeric Golgi (COG) complex, which plays a crucial role in maintaining Golgi apparatus function and protein glycosylation. Research into COG3 and its associated pathways has gained significant attention due to its involvement in various cellular processes, including membrane traffic, protein sorting, and the maintenance of Golgi structure. Mutations or dysfunctions in COG proteins, including COG3, are linked to congenital disorders, such as Congenital Disorders of Glycosylation (CDGs), highlighting their importance in human health. Studies have revealed that COG3 is essential for the retrograde transport of proteins from the Golgi back to the endoplasmic reticulum, contributing to the overall homeostasis and functionality of the secretory pathway. Additionally, COG3 is implicated in diverse biological processes, including cell differentiation, development, and immune responses, as its proper function is vital for the correct glycosylation of numerous glycoproteins. Ongoing research aims to elucidate the precise mechanisms by which COG3 interacts with other Golgi-associated proteins and molecules, and how these interactions may be disrupted in pathological conditions. Understanding the role of COG3 in cellular physiology may provide insights into therapeutic strategies for treating COG-related disorders and enhancing our general comprehension of Golgi function in eukaryotic cells.
