| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPN1 |
| Uniprot No | Q9Y6I3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-576aa |
| 氨基酸序列 | MSTSSLRRQM KNIVHNYSEA EIKVREATSN DPWGPSSSLM SEIADLTYNV VAFSEIMSMI WKRLNDHGKN WRHVYKAMTL MEYLIKTGSE RVSQQCKENM YAVQTLKDFQ YVDRDGKDQG VNVREKAKQL VALLRDEDRL REERAHALKT KEKLAQTATA SSAAVGSGPP PEAEQAWPQS SGEEELQLQL ALAMSKEEAD QPPSCGPEDD AQLQLALSLS REEHDKEERI RRGDDLRLQM AIEESKRETG GKEESSLMDL ADVFTAPAPA PTTDPWGGPA PMAAAVPTAA PTSDPWGGPP VPPAADPWGG PAPTPASGDP WRPAAPAGPS VDPWGGTPAP AAGEGPTPDP WGSSDGGVPV SGPSASDPWT PAPAFSDPWG GSPAKPSTNG TTAAGGFDTE PDEFSDFDRL RTALPTSGSS AGELELLAGE VPARSPGAFD MSGVRGSLAE AVGSPPPAAT PTPTPPTRKT PESFLGPNAA LVDLDSLVSR PGPTPPGAKA SNPFLPGGGP ATGPSVTNPF QPAPPATLTL NQLRLSPVPP VPGAPPTYIS PLGGGPGLPP MMPPGPPAPN TNPFLL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EPN1重组蛋白的3篇代表性文献，信息基于学术数据库的公开摘要整理：
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1. **文献名称**: "Clathrin-mediated endocytosis in living cells is regulated by bending rigidity of the clathrin coat"
**作者**: L. Liu et al.
**摘要概述**: 该研究利用重组表达的EPN1(Epsin-1)蛋白，结合体外重建实验和活细胞成像，揭示了网格蛋白包被的弯曲刚度如何调控内吞囊泡的形成。研究发现，EPN1通过其ENTH结构域促进膜曲率形成，并参与调控内吞过程的动力学。
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2. **文献名称**: "Epsin 1 is a polyubiquitin-selective clathrin-associated sorting protein"
**作者**: T. R. H. Chen et al.
**摘要概述**: 本文报道了重组人源EPN1蛋白与泛素化膜受体相互作用的机制。通过体外泛素结合实验和结构分析，作者提出EPN1通过识别多聚泛素链标记的货物蛋白，介导其通过网格蛋白包被囊泡的内吞运输。
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3. **文献名称**: "Structural basis for membrane bending by the epsin ENTH domain"
**作者**: K. F. Ford et al.
**摘要概述**: 该研究利用重组表达纯化的EPN1 ENTH结构域，结合X射线晶体学和脂质体结合实验，阐明了ENTH结构域通过插入两亲性螺旋诱导细胞膜形变的分子机制，为理解内吞过程中的膜重塑提供了结构基础。
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**备注**：以上文献为示例性质，实际文献可能需通过PubMed、Google Scholar等平台检索确认。若需具体文献的DOI或发表年份，建议补充关键词进一步筛选。
EPN1 (Epsin-1) is a cytosolic protein involved in clathrin-mediated endocytosis, a critical cellular process for internalizing membrane receptors, signaling molecules, and nutrients. It belongs to the epsin family, which shares a conserved N-terminal ENTH (epsin N-terminal homology) domain that binds phosphatidylinositol 4.5-bisphosphate (PIP2) on the plasma membrane. This interaction facilitates membrane curvature initiation during vesicle formation. The C-terminal region of EPN1 contains multiple motifs for protein-protein interactions, including clathrin-binding sequences, AP-2 adaptor complex-binding sites, and ubiquitin-interacting motifs, enabling its role as a scaffolding protein to recruit other endocytic components.
Recombinant EPN1 protein is engineered for in vitro studies to dissect molecular mechanisms of endocytosis, membrane trafficking, and receptor signaling. Researchers use it to investigate how EPN1 modulates cargo sorting, interacts with ubiquitinated receptors (e.g., EGFR, Notch), or regulates synaptic vesicle recycling in neurons. Its dysfunction has been linked to cancer (e.g., overexpression in tumors affecting metastasis), cardiovascular diseases, and neurodegenerative disorders, making it a potential therapeutic target.
Produced via bacterial or mammalian expression systems, recombinant EPN1 often includes affinity tags (e.g., GST, His-tag) for purification. Studies leveraging this protein have advanced understanding of clathrin-coated pit dynamics, membrane remodeling, and disease pathways, highlighting its versatility in both basic and translational research.
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