关键信息
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基因名
SH2D1B
- 应用
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别名
SH2D1B; EAT2; SH2 domain-containing protein 1B; EWS/FLI1-activated transcript 2; EAT-2
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O14796
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表达区间
1-132 aa
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氨基酸序列
MDLPYYHGRL TKQDCETLLL KEGVDGNFLL RDSESIPGVL CLCVSFKNIV YTYRIFREKH GYYRIQTAEG SPKQVFPSLK ELISKFEKPN QGMVVHLLKP IKRTSPSLRW RGLKLELETF VNSNSDYVDV LP
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分子量
15.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SH2D1B, also known as Src Homology 2 Domain Containing 1B, is a protein that plays a crucial role in immune signaling pathways, particularly in T-cell activation and function. It interacts with various signaling molecules, influencing cellular responses to stimuli. The study of SH2D1B is particularly relevant in the context of autoimmune diseases and immunological disorders, where dysregulation of T-cell signaling can lead to pathological conditions. Research has shown that SH2D1B is involved in the modulation of pathways such as the JAK-STAT signaling cascade, which is vital for the development and differentiation of immune cells. Furthermore, SH2D1B is linked to the genetic basis of certain immunodeficiencies, where mutations can impair its function, highlighting its importance in maintaining immune homeostasis. The production of recombinant SH2D1B is essential for detailed biochemical and biophysical characterization, allowing researchers to better understand its structure-function relationship. Such studies can pave the way for the development of therapeutic strategies aimed at correcting the signaling defects associated with SH2D1B dysfunction. Overall, the exploration of SH2D1B as a recombinant protein provides valuable insights into the fundamental mechanisms of immune regulation, with implications for therapeutic interventions in immune-related diseases.
