| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZFP2 |
| Uniprot No | Q6ZN57 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-461 aa |
| 活性数据 | MEREGIWHSTLGETWEPNNWLEGQQDSHLSQVGVTHKETFTEMRVCGGNEFERCSSQDSILDTQQSIPMVKRPHNCNSHGEDATQNSELIKTQRMFVGKKIYECNQCSKTFSQSSSLLKHQRIHTGEKPYKCNVCGKHFIERSSLTVHQRIHTGEKPYKCNECGKAFSQSMNLTVHQRTHTGEKPYQCKECGKAFHKNSSLIQHERIHTGEKPYKCNECGKAFTQSMNLTVHQRTHTGEKPYECNECGKAFSQSMHLIVHQRSHTGEKPYECSQCGKAFSKSSTLTLHQRNHTGEKPYKCNKCGKSFSQSTYLIEHQRLHSGVKPFECNECGKAFSKNSSLTQHRRIHTGEKPYECMVCGKHFTGRSSLTVHQVIHTGEKPYECNECGKAFSQSAYLIEHQRIHTGEKPYECDQCGKAFIKNSSLTVHQRTHTGEKPYQCNECGKAFSRSTNLTRHQRTHT |
| 分子量 | 79.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人ZFP2蛋白的参考文献(模拟整理，供参考)：
---
1. **文献名称**：*Role of ZFP2 in transcriptional repression and stem cell differentiation*
**作者**：Lee H, et al.
**摘要**：研究通过重组表达人ZFP2蛋白，发现其KRAB结构域能够招募表观遗传修饰复合物(如NuRD)，抑制靶基因转录，并在胚胎干细胞分化中调控多能性基因沉默。
---
2. **文献名称**：*ZFP2 regulates trophoblast development through epigenetic silencing*
**作者**：Chen R, et al.
**摘要**：利用重组人ZFP2蛋白进行染色质免疫沉淀(ChIP)分析，发现其在胎盘发育中通过靶向结合印记基因启动子，调控DNA甲基化和组蛋白修饰，影响滋养层细胞分化。
---
3. **文献名称**：*Structural insights into ZFP2-DNA binding specificity*
**作者**：Smith J, et al.
**摘要**：通过X射线晶体学解析了重组人ZFP2蛋白的锌指结构域与DNA复合物结构，阐明了其识别特定GC-rich序列的分子机制，为设计ZFP人工转录因子奠定基础。
---
4. **文献名称**：*ZFP2 as a potential biomarker in cancer progression*
**作者**：Wang Y, et al.
**摘要**：重组人ZFP2蛋白功能实验表明，其在乳腺癌细胞中通过抑制肿瘤抑制基因(如p53)的增强子活性，促进上皮-间质转化(EMT)，提示其作为癌症治疗靶点的潜力。
---
**说明**：以上文献为示例，实际研究中需根据具体数据库(如PubMed、Web of Science)验证准确性。重组ZFP2相关研究多聚焦于表观遗传调控、发育或疾病机制。
Zinc finger protein 2 (ZFP2), a member of the zinc finger protein family, plays crucial roles in transcriptional regulation and chromatin remodeling. Characterized by conserved Cys₂His₂ (C2H2) zinc finger motifs, ZFP2 binds DNA through these domains, enabling sequence-specific interactions with target genes. It is involved in diverse biological processes, including cell differentiation, embryonic development, and apoptosis. Studies suggest ZFP2 participates in epigenetic regulation by recruiting histone-modifying enzymes or influencing chromatin accessibility, thereby modulating gene expression profiles.
Recombinant human ZFP2 protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional studies. Its production involves cloning the ZFP2 gene into expression vectors, followed by purification via affinity chromatography. The recombinant protein retains DNA-binding activity, making it valuable for in vitro assays, such as electrophoretic mobility shift assays (EMSAs) or chromatin immunoprecipitation (ChIP) simulations.
Aberrant ZFP2 expression has been linked to diseases, including cancers and autoimmune disorders, highlighting its potential as a therapeutic target. Research on recombinant ZFP2 aids in elucidating its molecular mechanisms and exploring applications in gene editing or epigenetic therapy. However, its full functional spectrum and regulatory networks remain under investigation, necessitating further studies to unravel its context-dependent roles in health and disease.
×
