Cat: PA2000-3214

Recombinant Human CHD1L Protein,His

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关键信息

  • 基因名

    CHD1L

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    CHD1L;ALC1;Chromodomain-helicase-DNA-binding Protein 1-like

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    Q86WJ1

  • 表达区间

    704-897aa

  • 氨基酸序列

    SAELDYQDPDATSLKYVSGDVTHPQAGAEDALIVHCVDDSGHWGRGGLFTALEKRSAEPRKIYELAGKMKDLSLGGVLLFPVDDKESRNKGQDLLALIVAQHRDRSNVLSGIKMAALEEGLKKIFLAAKKKKASVHLPRIGHATKGFNWYGTERLIRKHLAARGIPTYIYYFPRSKSAVLHAQSSSSSSRQLVP

  • 分子量

    37.3 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

CHD1L (Chromodomain Helicase DNA-Binding Protein 1-Like) is a member of the chromodomain helicase DNA-binding (CHD) family, which plays a crucial role in diverse cellular processes, including chromatin remodeling, gene regulation, and DNA repair. Recent studies have suggested that CHD1L is involved in various biological functions, particularly in cancer biology, where its expression levels are often associated with tumor progression and poor prognosis. Understanding the structure and function of recombinant CHD1L protein is essential for elucidating its role in these processes. Researchers have focused on producing recombinant CHD1L to investigate its interactions with DNA and other proteins, as well as its potential regulatory mechanisms. Initial experiments have shown that CHD1L possesses ATPase activity, which may contribute to its chromatin remodeling functions. Furthermore, there is ongoing research into its implications in specific types of cancer, such as breast and colorectal cancer, where aberrant expression patterns of CHD1L have been observed. Given its potential as a therapeutic target, further investigation into the biochemical properties and molecular mechanisms of CHD1L is vital. Understanding how this protein operates within the cellular context could pave the way for novel therapeutic strategies aimed at modulating its activity, offering hope for improved cancer treatments and better outcomes for patients. Overall, the study of recombinant CHD1L represents a significant step toward unlocking the complexities of chromatin dynamics and their impact on human health.

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