关键信息
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基因名
PLD4
- 应用
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别名
PLD4；C14orf175；5'-3' exonuclease PLD4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96BZ4
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表达区间
52-506aa
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氨基酸序列
WQVPRPPTWGQVQPKDVPRSWEHGSSPAWEPLEAEARQQRDSCQLVLVESIPQDLPSAAGSPSAQPLGQAWLQLLDTAQESVHVASYYWSLTGPDIGVNDSSSQLGEALLQKLQQLLGRNISLAVATSSPTLARTSTDLQVLAARGAHVRQVPMGRLTRGVLHSKFWVVDGRHIYMGSANMDWRSLTQVKELGAVIYNCSHLAQDLEKTFQTYWVLGVPKAVLPKTWPQNFSSHFNRFQPFHGLFDGVPTTAYFSASPPALCPQGRTRDLEALLAVMGSAQEFIYASVMEYFPTTRFSHPPRYWPVLDNALRAAAFGKGVRVRLLVGCGLNTDPTMFPYLRSLQALSNPAANVSVDVKVFIVPVGNHSNIPFSRVNHSKFMVTEKAAYIGTSNWSEDYFSSTAGVGLVVTQSPGAQPAGATVQEQLRQLFERDWSSRYAVGLDGQAPGQDCVWQG
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分子量
66.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PLD4 (phospholipase D4) is a member of the phospholipase D family, which plays critical roles in various cellular processes, including membrane trafficking, signal transduction, and inflammation. Emerging studies have linked PLD4 to processes such as lipid metabolism and immune response, making it a potential target for therapeutic intervention in immune-related disorders and cancer. Research indicates that PLD4 is involved in the generation of phosphatidic acid from phosphatidylcholine, a key signaling molecule that regulates cell growth and differentiation. Furthermore, mutations or alterations in PLD4 expression have been associated with several diseases, including autoimmune conditions and certain types of cancer, highlighting its significance in health and disease. Understanding the structure, enzymatic activity, and regulatory mechanisms of PLD4 is crucial for elucidating its biological functions and potential as a drug target. Recent advancements in recombinant protein technology have enabled the production of functional PLD4 proteins, facilitating in-depth studies of its biochemical properties and interaction partners. Investigating PLD4's role in cellular pathways is expected to provide insights into its contributions to both normal physiology and pathological conditions, opening avenues for novel therapeutic strategies aimed at modulating its activity in disease contexts. As such, PLD4 represents a compelling focus for ongoing and future research in the field of molecular biology and pharmacology.
