关键信息
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基因名
SLC4A4
- 应用
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别名
DKFZp781H1314; Electrogenic sodium bicarbonate cotransporter 1; hhNMC; HNBC 1; HNBC1; kNBC 1; KNBC; kNBC1; Na(+)/HCO3(-) cotransporter; Na+HCO3- cotransporter 4; NBC 1; NBC 2; NBC1; NBC2; Nbc4; NBCE 1; NBCE1; OTTHUMP00000160355; OTTHUMP00000218884; OTTHUMP00000218885; PNBC; S4A4_HUMAN; SLC4A4; SLC4A5; Sodium bicarbonate cotransporter 1 (sodium bicarbonate cotransporter. kidney. sodium bicarbonate cotransporter. pancreas); Sodium bicarbonate cotransporter; Sodium bicarbonate cotransporter kidney; sodium bicarbonate cotransporter member 4; Sodium bicarbonate cotransporter pancreas; Solute carrier family 4 member 4; Solute carrier family 4 sodium bicarbonate cotransporter member 4; Solute carrier family 4 sodium bicarbonate cotransporter member 5; Solute carrier family 4. sodium bicarbonate cotransporter. member 4. brain type
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y6R1
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表达区间
1-646 aa
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氨基酸序列
MSTENVEGKPSNLGERGRARSSTFLRVVQPMFNHSIFTSAVSPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFTNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDNNFDYLEFRLWIGLWSAFLCLILVATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSNFKVGYNTLFSCTCVPPDPGEGITLCVYARFVFGGRCRLHACKFSTCCHGPQELVLFFSLKNSATEFDVSLPEVF
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分子量
96.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SLC4A4, also known as the Sodium Bicarbonate Cotransporter 1 (NBC1), is a crucial membrane protein that plays a key role in the regulation of bicarbonate transport across cell membranes, contributing to various physiological processes such as acid-base homeostasis and cell volume regulation. Abnormalities in SLC4A4 function have been linked to several pathophysiological conditions, including metabolic alkalosis and developmental issues related to the central nervous system. The exploration of SLC4A4 as a recombinant protein has become increasingly important in understanding its structure-function relationships, mechanisms of action, and potential therapeutic applications. Techniques like gene cloning and protein purification enable researchers to produce functional SLC4A4 for biochemical and biophysical studies. Investigating SLC4A4’s interactions with other proteins and its role in cellular responses to environmental changes has significant implications for drug design and development. Moreover, enhanced knowledge of SLC4A4 can lead to better-targeted therapies for conditions resulting from its dysregulation, including renal disorders and neurological diseases.
