关键信息
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基因名
CYP2J2
- 应用
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别名
Albendazole monooxygenase hydroxylating;Albendazole monooxygenase sulfoxide-forming;Arachidonic acid epoxygenase;CYPIIJ2;Hydroperoxy icosatetraenoate isomerase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P51589
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表达区间
33-502aa
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氨基酸序列
DFLKRRRPKNYPPGPWRLPFLGNFFLVDFEQSHLEVQLFVKKYGNLFSLELGDISAVLITGLPLIKEALIHMDQNFGNRPVTPMREHIFKKNGLIMSSGQAWKEQRRFTLTALRNFGLGKKSLEERIQEEAQHLTEAIKEENGQPFDPHFKINNAVSNIICSITFGERFEYQDSWFQQLLKLLDEVTYLEASKTCQLYNVFPWIMKFLPGPHQTLFSNWKKLKLFVSHMIDKHRKDWNPAETRDFIDAYLKEMSKHTGNPTSSFHEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEIQEKVQAEIDRVIGQGQQPSTAARESMPYTNAVIHEVQRMGNIIPLNVPREVTVDTTLAGYHLPKGTMILTNLTALHRDPTEWATPDTFNPDHFLENGQFKKREAFMPFSIGKRACLGEQLARTELFIFFTSLMQKFTFRPPNNEKLSLKFRMGITISPVSHRLCAVPQV
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分子量
61.3 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CYP2J2 is a member of the cytochrome P450 superfamily of enzymes, primarily found in the human liver and heart, known for its role in the metabolism of various endogenous and exogenous compounds. This enzyme catalyzes the oxidation of long-chain fatty acids and plays a significant role in the bioactivation of certain drugs, as well as the metabolism of the therapeutic agent Sunitinib. The functional significance of CYP2J2 extends beyond drug metabolism, as it is involved in the production of epoxyeicosatrienoic acids (EETs), which possess vasodilatory, anti-inflammatory, and cardioprotective effects. Dysfunction or altered expression of CYP2J2 has been linked to various cardiovascular diseases, making it a potential biomarker for cardiovascular health and a target for therapeutic interventions. Recent studies have focused on the recombinant expression of CYP2J2 to facilitate the characterization of its enzymatic activity and substrate specificity. These efforts aim to understand the impact of genetic polymorphisms and regulatory mechanisms that modify CYP2J2 function, ultimately contributing to advancements in precision medicine and personalized therapy strategies. The recombinant protein studies not only enhance our understanding of CYP2J2's pharmacogenomic relevance but also pave the way for innovative drug development approaches targeting CYP2J2's metabolic pathways.
