关键信息
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基因名
CREB
- 应用
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别名
CREB；Cyclic AMP-responsive element-binding Protein 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P16220
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表达区间
1-341aa
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氨基酸序列
MTMESGAENQ QSGDAAVTEA ENQQMTVQAQ PQIATLAQVS MPAAHATSSA PTVTLVQLPN GQTVQVHGVI QAAQPSVIQS PQVQTVQSSC KDLKRLFSGT QISTIAESED SQESVDSVTD SQKRREILSR RPSYRKILND LSSDAPGVPR IEEEKSEEET SAPAITTVTV PTPIYQTSSG QYIAITQGGA IQLANNGTDG VQGLQTLTMT NAAATQPGTT ILQYAQTTDG QQILVPSNQV VVQAASGDVQ TYQIRTAPTS TIAPGVVMAS SPALPTQPAE EAARKREVRL MKNREAAREC RRKKKEYVKC LENRVAVLEN QNKTLIEELK ALKDLYCHKS D
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CREB (cAMP Response Element-Binding protein) is a transcription factor that plays a crucial role in regulating gene expression in response to various extracellular signals, particularly those mediated by cyclic AMP (cAMP). It is involved in numerous physiological processes, including energy metabolism, neuroplasticity, and cell survival. The CREB protein functions by binding to specific DNA sequences known as cAMP response elements, facilitating the transcription of target genes associated with learning, memory, and stress responses. Dysregulation of CREB has been implicated in several pathologies, such as depression, addiction, and neurodegenerative diseases. Consequently, understanding the structure and function of CREB, particularly through recombinant protein studies, has become a focal point of research. Recombinant CREB proteins, produced through advanced genetic engineering techniques, allow scientists to dissect the molecular mechanisms underlying CREB's activity, elucidate its interactions with other proteins and nucleic acids, and assess the effects of mutations or post-translational modifications. This research not only enhances our comprehension of CREB's role in cell signaling pathways but also holds potential for therapeutic strategies aimed at modulating CREB activity for the treatment of related disorders.
