Cat: PA2000-3378

Recombinant Human MTIF3 Protein,His

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关键信息

  • 基因名

    MTIF3

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    MTIF3;Translation initiation factor IF-3. mitochondrial

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    Q9H2K0

  • 表达区间

    1-278aa

  • 氨基酸序列

    TAPAQLSPIASAPRLSFLIHAKAFSTAEDTQNEGKKTKKNKTAFSNVGRKISQRVIHLFDEKGNDLGNMHRANVIRLMDERDLRLVQRNTSTEPAEYQLMTGLQILQERQRLREMEKANPKTGPTLRKELILSSNIGQHDLDTKTKQIQQWIKKKHLVQITIKKGKNVDVSENEMEEIFHQILQTMPGIATFSSRPQAVQGGKALMCVLRAFSKNEEKAYKETQETQERDTLNKDHGNDKESNVLHQ

  • 分子量

    55.2 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

MTIF3 (Mitochondrial Translation Initiation Factor 3) is a crucial protein involved in the initiation of mitochondrial translation, playing a significant role in the proper synthesis of mitochondrial-encoded proteins. These proteins are essential for various mitochondrial functions, including energy production, oxidative phosphorylation, and overall cellular metabolism. Research on MTIF3 has gained importance due to its implications in mitochondrial diseases, which can arise from mutations affecting mitochondrial translation. Defective mitochondrial protein synthesis can lead to a cascade of metabolic dysfunctions, resulting in conditions that may manifest as neurodegenerative disorders, myopathies, and metabolic syndromes. Understanding the structure and function of MTIF3 can provide insights into the mechanisms of mitochondrial protein synthesis and the potential link to pathogenic processes. Moreover, MTIF3 may serve as a valuable biomarker for mitochondrial dysfunction and a target for therapeutic strategies aimed at treating mitochondrial diseases. Recent advances in molecular biology techniques, including cryo-electron microscopy and high-throughput sequencing, have facilitated a deeper investigation into the dynamics of mitochondrial translation and the role of factors like MTIF3. Thus, ongoing research in this area holds promise for uncovering novel therapeutic avenues for alleviating the burden of mitochondrial-related disorders and enhancing our understanding of mitochondrial biology.

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