| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ASK1 |
| Uniprot No | Q99683 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1374aa |
| 氨基酸序列 | MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT |
| 预测分子量 | 154,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ASK1重组蛋白的3篇代表性文献，供参考：
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1. **文献名称**：*Induction of Apoptosis by ASK1. a Mammalian MAPKKK That Activates SAPK/JNK and p38 Signaling Pathways*
**作者**：Ichijo H. et al.
**摘要**：该研究首次报道了ASK1重组蛋白的克隆与功能，证实其在氧化应激诱导的细胞凋亡中通过激活JNK/p38信号通路发挥关键作用，并揭示了其与TRAF2的相互作用机制。
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2. **文献名称**：*ASK1 is essential for JNK/SAPK activation by TRAF2*
**作者**：Nishitoh H. et al.
**摘要**：文章利用重组ASK1蛋白验证了其在TNF-α信号通路中的作用，证明ASK1是TRAF2下游激活JNK信号的必要激酶，并参与炎症反应和细胞死亡调控。
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3. **文献名称**：*Reactive oxygen species promote TNFα-induced death through sustained activation of ASK1/JNK*
**作者**：Tobiume K. et al.
**摘要**：研究通过体外重组蛋白实验，阐明活性氧(ROS)通过抑制ASK1与抑制蛋白(如Thioredoxin)的结合，持续激活ASK1-JNK通路，进而促进TNFα诱导的细胞凋亡。
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**备注**：以上文献均发表于*Science*、*EMBO Journal*等权威期刊，聚焦于ASK1重组蛋白在信号通路、疾病机制中的功能验证。如需具体发表年份或卷期，可进一步补充。
**Background of ASK1 Recombinant Protein**
Apoptosis signal-regulating kinase 1 (ASK1), also known as MAP3K5. is a serine/threonine protein kinase belonging to the mitogen-activated protein kinase kinase kinase (MAP3K) family. It plays a pivotal role in stress-induced cellular signaling pathways, particularly in response to oxidative stress, endoplasmic reticulum (ER) stress, and inflammatory cytokines. ASK1 is activated by diverse stimuli, including reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), and calcium overload, which trigger its autophosphorylation and subsequent activation of downstream MAPK pathways, such as p38 and JNK. These pathways regulate critical cellular processes, including apoptosis, differentiation, and inflammation.
Structurally, ASK1 contains an N-terminal inhibitory domain, a central kinase domain, and a C-terminal regulatory domain. Under basal conditions, ASK1 is kept inactive through interactions with inhibitory proteins like thioredoxin (Trx). However, under stress conditions, Trx dissociates, enabling ASK1 oligomerization and activation. Dysregulation of ASK1 signaling has been implicated in various diseases, including neurodegenerative disorders, cardiovascular diseases, cancer, and metabolic syndromes, highlighting its therapeutic potential.
Recombinant ASK1 protein is engineered for research applications, produced using expression systems like *E. coli* or mammalian cells. It retains functional kinase activity and is widely used to study ASK1-mediated signaling *in vitro*, screen inhibitors, or elucidate structural and mechanistic details. Its production enables targeted exploration of ASK1's role in disease pathways and the development of kinase-specific therapeutics.
In summary, ASK1 recombinant protein serves as a vital tool for dissecting stress-responsive signaling mechanisms and advancing drug discovery efforts aimed at modulating ASK1-associated pathologies.
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