关键信息
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基因名
KLF5
- 应用
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别名
KLF5；BTEB2；CKLF；IKLF；Krueppel-like factor 5
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13887
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表达区间
1-457aa
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氨基酸序列
MATRVLSMSA RLGPVPQPPA PQDEPVFAQL KPVLGAANPA RDAALFPGEE LKHAHHRPQA QPAPAQAPQP AQPPATGPRL PPEDLVQTRC EMEKYLTPQL PPVPIIPEHK KYRRDSASVV DQFFTDTEGL PYSINMNVFL PDITHLRTGL YKSQRPCVTH IKTEPVAIFS HQSETTAPPP APTQALPEFT SIFSSHQTAA PEVNNIFIKQ ELPTPDLHLS VPTQQGHLYQ LLNTPDLDMP SSTNQTAAMD TLNVSMSAAM AGLNTHTSAV PQTAVKQFQG MPPCTYTMPS QFLPQQATYF PPSPPSSEPG SPDRQAEMLQ NLTPPPSYAA TIASKLAIHN PNLPTTLPVN SQNIQPVRYN RRSNPDLEKR RIHYCDYPGC TKVYTKSSHL KAHLRTHTGE KPYKCTWEGC DWRFARSDEL TRHYRKHTGA KPFQCGVCNR SFSRSDHLAL HMKRHQN
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KLF5 (Kruppel-like factor 5) is a crucial transcription factor belonging to the KLF family, which plays a significant role in various biological processes, including cell differentiation, proliferation, and apoptosis. Its involvement in regulating gene expression has made it a focal point of research, particularly in relation to stem cell biology and cancer. KLF5 is known to be overexpressed in several types of cancer, including breast and colorectal cancers, highlighting its potential as a therapeutic target. The study of recombinant KLF5 protein is essential for understanding its functional mechanisms and interactions at the molecular level. Researchers are increasingly focusing on the expression and purification of KLF5 protein using recombinant DNA technology, which allows for the production of large amounts of the protein necessary for biochemical assays and structural studies. These investigations aim to elucidate KLF5's role in tumorigenesis and its interaction with other proteins and DNA, providing insights into its potential as a biomarker for cancer diagnosis and a target for novel therapeutic approaches. Overall, the research surrounding recombinant KLF5 protein is pivotal for advancing our understanding of its contributions to both normal physiological processes and pathological conditions.
