| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NBC4 |
| Uniprot No | Q9BY07 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1137aa |
| 氨基酸序列 | MKVKEEKAGVGKLDHTNHRRRFPDQKECPPIHIGLPVPTYPQRKTDQKGHLSGLQKVHWGLRPDQPQQELTGPGSGASSQDSSMDLISRTRSPAAEQLQDILGEEDEAPNPTLFTEMDTLQHDGDQMEWKESARWIKFEEKVEEGGERWSKPHVSTLSLHSLFELRTCLQTGTVLLDLDSGSLPQIIDDVIEKQIEDGLLRPELRERVSYVLLRRHRHQTKKPIHRSLADIGKSVSTTNRSPARSPGAGPSLHHSTEDLRMRQSANYGRLCHAQSRSMNDISLTPNTDQRKNKFMKKIPKDSEASNVLVGEVDFLDQPFIAFVRLIQSAMLGGVTEVPVPTRFLFILLGPSGRAKSYNEIGRAIATLMVDDLFSDVAYKARNREDLIAGIDEFLDEVIVLPPGEWDPNIRIEPPKKVPSADKRKSVFSLAELGQMNGSVGGGGGAPGGGNGGGGGGGSGGGAGSGGAGGTSSGDDGEMPAMHEIGEELIWTGRFFGGLCLDIKRKLPWFPSDFYDGFHIQSISAILFIYLGCITNAITFGGLLGDATDNYQGVMESFLGTAMAGSLFCLFSGQPLIILSSTGPILIFEKLLFDFSKGNGLDYMEFRLWIGLHSAVQCLILVATDASFIIKYITRFTEEGFSTLISFIFIYDAIKKMIGAFKYYPINMDFKPNFITTYKCECVAPDTVNTTVFNASAPLAPDTNASLYNLLNLTALDWSLLSKKECLSYGGRLLGNSCKFIPDLALMSFILFFGTYSMTLTLKKFKFSRYFPTKVRALVADFSIVFSILMFCGIDACFGLETPKLHVPSVIKPTRPDRGWFVAPFGKNPWWVYPASILPALLVTILIFMDQQITAVIVNRKENKLKKAAGYHLDLFWVGILMALCSFMGLPWYVAATVISIAHIDSLKMETETSAPGEQPQFLGVREQRVTGIIVFILTGISVFLAPILKCIPLPVLYGVFLYMGVASLNGIQMGTGGSEFKIQKKLTPFWERCKLFLMPAKHQPDHAFLRHVPLRRIHLFTLVQILCLAVLWILKSTVAAIIFPVMILGLIIVRRLLDFIFSQHDLAWIDNILPEKEKKETDKKRKRKKGAHEDCDEEPQFPPPSVIKIPMESVQSDPQNGIHCIARKRSSSWSYSL |
| 预测分子量 | 126,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NBC4重组蛋白的模拟参考文献示例，供参考：
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1. **文献名称**：*Functional Analysis of Recombinant NBC4 in pH Regulation of Epithelial Cells*
**作者**：Lee J, et al.
**摘要**：本研究通过在大肠杆菌和哺乳动物细胞中表达重组NBC4蛋白，揭示了其在调节上皮细胞胞内pH中的作用。结果显示，NBC4通过介导HCO₃⁻跨膜转运维持酸碱平衡，并受钙离子信号通路的调控。
2. **文献名称**：*Cryo-EM Structure Determination of Human NBC4 Reveals Transport Mechanism*
**作者**：Wang H, et al.
**摘要**：利用重组人源NBC4蛋白的纯化与冷冻电镜技术，首次解析了其高分辨率三维结构，阐明了底物结合位点及离子转运的分子机制，为靶向药物开发提供了结构基础。
3. **文献名称**：*Role of Recombinant NBC4 in Ischemic Kidney Injury*
**作者**：Gomez S, et al.
**摘要**：通过构建NBC4重组蛋白的体外模型，发现其在肾小管细胞缺血再灌注损伤中通过调节细胞凋亡和氧化应激发挥保护作用，提示其作为治疗急性肾损伤的潜在靶点。
4. **文献名称**：*Kinetic Properties of Recombinant NBC4 in Xenopus laevis Oocytes*
**作者**：Tanaka K, et al.
**摘要**：在非洲爪蟾卵母细胞中表达重组NBC4.结合电生理学方法测定其转运动力学参数，发现其活性受膜电位及Na⁺浓度梯度的影响，为理解生理条件下的功能提供了数据支持。
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**注**：以上文献为示例，实际研究中建议通过学术数据库(如PubMed、Web of Science)检索具体文献。若需进一步精准查找，可结合关键词“NBC4 recombinant protein”“SLC4A4 expression”等。
NBC4 (electrogenic sodium bicarbonate cotransporter 1), encoded by the *SLC4A4* gene, is a membrane transport protein critical for maintaining pH homeostasis and bicarbonate (HCO₃⁻) transport in various tissues. As a member of the solute carrier family 4 (SLC4), NBC4 mediates the coupled movement of sodium (Na⁺) and bicarbonate ions across cellular membranes, primarily functioning in an electrogenic manner (1:3 stoichiometry of Na⁺:HCO₃⁻). It is expressed in organs such as the kidneys, heart, and brain, where it regulates acid-base balance, cellular metabolism, and ion flux.
Recombinant NBC4 protein is produced using biotechnological methods, typically through heterologous expression in mammalian cell lines (e.g., HEK293) or insect cells via baculovirus systems. The recombinant protein retains the structural and functional properties of the native transporter, enabling in vitro studies on its kinetics, regulation, and interactions. Researchers often purify the protein using affinity tags (e.g., His-tag) and validate its activity via radiolabeled ion flux assays or electrophysiological measurements.
NBC4 dysfunction is linked to diseases like proximal renal tubular acidosis (pRTA) and glaucoma, driving interest in its recombinant form for mechanistic studies. Recombinant NBC4 facilitates investigations into mutations affecting transporter activity, drug screening for therapeutic modulators, and structural analyses (e.g., cryo-EM) to elucidate its conformational dynamics. Its role in cancer cell survival under acidic microenvironments has also spurred oncology-related research. By providing a controlled, scalable source of functional protein, recombinant NBC4 accelerates both basic research and translational applications targeting bicarbonate-dependent physiological processes.
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