关键信息
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基因名
GRIN1
- 应用
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别名
GRIN1；KIAA1893；G Protein-regulated inducer of neurite outgrowth 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q05586
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表达区间
834-938aa
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氨基酸序列
EIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKATFRAITSTLASSFKRRRSSKDTSTGGGRGALQNQKDTVLPRRAIEREEGQLQLCSRHRES
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分子量
18.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
GRIN1, a gene that encodes the GluN1 subunit of the NMDA receptor, plays a crucial role in neurotransmission and synaptic plasticity in the brain. Mutations in GRIN1 have been associated with various neurological disorders, including epilepsy, intellectual disability, and developmental delay, highlighting its importance in neural function. The NMDA receptor, a key player in excitatory neurotransmission, is involved in critical processes such as learning and memory. Understanding the structure and function of GRIN1 recombinantly expressed proteins can provide insights into the receptor's mechanisms and its pathology in related disorders. Researchers utilize recombinant GRIN1 proteins to study ligand binding, receptor activation, and signaling pathways, opening pathways for therapeutic interventions. Additionally, these recombinant proteins serve as valuable tools for drug screening and the development of small molecules targeting dysfunctional NMDA receptors. Overall, the investigation of GRIN1 recombinant protein is pivotal for advancing our comprehension of excitatory neurotransmission and developing strategies to address GRIN1-related diseases.
