关键信息
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基因名
SRRM4
- 应用
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别名
SRRM4; KIAA1853; Serine/arginine repetitive matrix protein 4; Medulloblastoma antigen MU-MB-2.76; Neural-specific serine/arginine repetitive splicing factor of 100 kDa; Neural-specific SR-related protein of 100 kDa; nSR100
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
A7MD48
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表达区间
1-611aa
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氨基酸序列
MASVQQGEKQ LFEKFWRGTF KAVATPRPES IIVASITARK PLPRTEPQNN PVVPAQDGPS EKLGQHLATE PLGTNSWERD KTCRELGATR GHSASHDKDL TPPPSSRGKK KKKKSTRKKR RRSSSYSPSP VKKKKKKSSK KHKRRRSFSK KRRHSSSSPK SKRRDEKRHK KQSRSRPRKS HRHRHHRCPS RSQSSESRPS SCESRHRGRS PEEGQKSRRR HSRRCSKTLC KDSPEAQSSR PPSQPLQMLG YLSARGVITG SGSAADLFTK TASPLTTSRG RSQEYDSGND TSSPPSTQTS SARSRGQEKG SPSGGLSKSR ELNSGNTSDS GNSFTTSSPQ NKGAMLENLS PTSRGRESRG FQSPCLECAE VKKSSLVPST ARSSPMKGCS RSSSYASTRS SSHSSRSPNP RASPRYTQSR STSSEKRSYS RSPSYSSKSG KRSPPSRSSR SRRSPSYSRY SPSRERDPKY SEKDSQQRER ERARRRRRSY SPMRKRRRDS PSHLEARRIT SARKRPIPYY RPSPSSSGSL SSTSSWYSSS SSRSASRSYS RSRSRSRSRR RSRTRTSSSS SSRSPSPGSR SRSRSRSRSR SRSRSQSRSY SSADSYSSTR R
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分子量
68.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
SRRM4 (Serine/Arginine-Rich Splicing Factor 4) is a pivotal protein involved in RNA splicing, a critical cellular process that determines the synthesis of functional proteins by removing introns from pre-mRNA. This protein plays a significant role in the regulation of alternative splicing, allowing a single gene to produce multiple protein isoforms, which can vastly contribute to the complexity of gene expression and cellular functions. Dysregulation of SRRM4 has been implicated in various diseases, including cancer and neurodegenerative disorders, where altered splicing patterns often lead to aberrant cellular behavior. The study of SRRM4 encompasses its molecular mechanisms, interactions with other splicing factors, and its influence on the splicing of specific target pre-mRNAs. Recombinant SRRM4 protein production is essential for elucidating its functional dynamics and developing potential therapeutic strategies. Researchers utilize techniques such as recombinant DNA technology to express and purify SRRM4 for biochemical studies, structural analysis, and functional assays. Understanding the structure-function relationship of SRRM4 could provide insights into its role in normal physiology and disease states, paving the way for novel interventions targeting aberrant splicing mechanisms in various pathologies. Overall, the investigation of SRRM4 and its recombinant protein form represents a significant endeavor in the field of molecular biology and therapeutic development.
