关键信息
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基因名
STX3A
- 应用
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别名
FLJ30906; Stx3; STX3_HUMAN; STX3A; Syntaxin 3A ; Syntaxin-3
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13277
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表达区间
1-289 aa
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氨基酸序列
MKDRLEQLKAKQLTQDDDTDAVEIAIDNTAFMDEFFSEIEETRLNIDKISEHVEEAKKLYSIILSAPIPEPKTKDDLEQLTTEIKKRANNVRNKLKSMEKHIEEDEVRSSADLRIRKSQHSVLSRKFVEVMTKYNEAQVDFRERSKGRIQRQLEITGKKTTDEELEEMLESGNPAIFTSGIIDSQISKQALSEIEGRHKDIVRLESSIKELHDMFMDIAMLVENQGEMLDNIELNVMHTVDHVEKARDETKKAVKYQSQARKKLIIIIVLVVVLLGILALIIGLSVGLN
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分子量
57.42 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
STX3A (Syntaxin 3A) is a member of the SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein REceptor) protein family, which plays a crucial role in vesicle trafficking and membrane fusion in eukaryotic cells. Recent research has highlighted the importance of STX3A in various cellular processes, including exocytosis, endocytosis, and neurotransmitter release, making it a significant focus in both basic and applied biology. Mutations or dysregulation of STX3A have been associated with several neurological disorders, underscoring its potential as a therapeutic target. The recombinant production of STX3A protein allows researchers to investigate its structure-function relationships, interactions with other SNARE proteins, and role in cellular dynamics at a molecular level. Utilizing advanced techniques such as X-ray crystallography and cryo-electron microscopy, researchers aim to unravel the intricate mechanisms by which STX3A mediates membrane fusion events. Furthermore, the study of STX3A in model organisms and neuronal cell lines offers insights into its physiological relevance and the pathways involved in synaptic transmission. Overall, the ongoing research on STX3A recombinant protein not only enriches our understanding of fundamental cellular processes but also opens avenues for the development of innovative therapeutic strategies for diseases associated with vesicle transport dysfunctions.
