关键信息
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基因名
TAF1D
- 应用
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别名
TAF1D; JOSD3; TATA box-binding protein-associated factor RNA polymerase I subunit D; RNA polymerase I-specific TBP-associated factor 41 kDa; TAFI41; TATA box-binding protein-associated factor 1D; TBP-associated factor 1D; Transcription initiation factor SL1/TIF-IB subunit D
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9H5J8
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表达区间
1-278 aa
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氨基酸序列
MDKSGIDSLD HVTSDAVELA NRSDNSSDSS LFKTQCIPYS PKGEKRNPIR KFVRTPESVH ASDSSSDSSF EPIPLTIKAI FERFKNRKKR YKKKKKRRYQ PTGRPRGRPE GRRNPIYSLI DKKKQFRSRG SGFPFLESEN EKNAPWRKIL TFEQAVARGF FNYIEKLKYE HHLKESLKQM NVGEDLENED FDSRRYKFLD DDGSISPIEE STAEDEDATH LEDNECDIKL AGDSFIVSSE FPVRLSVYLE EEDITEEAAL SKKRATKAKN TGQRGLKM
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分子量
32.0kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
TAF1D, a subunit of the transcription factor TFIID, plays a crucial role in the initiation of gene transcription by RNA polymerase II. This protein is part of a larger complex that recognizes and binds to the promoter regions of genes, facilitating the recruitment of other essential transcription machinery. Recent studies have highlighted the significance of TAF1D in various biological processes, including cell differentiation, proliferation, and response to stress. Its dysregulation has been implicated in several diseases, particularly cancer, making it a target of interest for therapeutic interventions. The recombinant expression of TAF1D in various systems, such as bacterial, yeast, or mammalian cells, enables researchers to analyze its structure-function relationship, post-translational modifications, and interactions with other proteins. By studying TAF1D at a molecular level, researchers aim to uncover the regulatory mechanisms underlying transcriptional control, providing insights that could lead to novel strategies for targeting gene expression in diseases characterized by aberrant transcriptional regulation. The development of effective recombinant proteins is vital for elucidating the functional dynamics of TAF1D and its role in cellular processes, paving the way for future studies and potential clinical applications.
