| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBLN7 |
| Uniprot No | Q53RD9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-439aa |
| 氨基酸序列 | QNCLSKQQLLSAIRQLQQLLKGQETRFAEGIRHMKSRLAALQNSVGRVGPDALPVSCPALNTPADGRKFGSKYLVDHEVHFTCNPGFRLVGPSSVVCLPNGTWTGEQPHCRGISECSSQPCQNGGTCVEGVNQYRCICPPGRTGNRCQHQAQTAAPEGSVAGDSAFSRAPRCAQVERAQHCSCEAGFHLSGAAGDSVCQDVNECELYGQEGRPRLCMHACVNTPGSYRCTCPGGYRTLADGKSCEDVDECVGLQPVCPQGTTCINTGGSFQCVSPECPEGSGNVSYVKTSPFQCERNPCPMDSRPCRHLPKTISFHYLSLPSNLKTPITLFRMATASAPGRAGPNSLRFGIVGGNSRGHFVMQRSDRQTGDLILVQNLEGPQTLEVDVDMSEYLDRSFQANHVSKVTIFVSPYDF |
| 预测分子量 | 51.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FBLN7(Fibulin-7)重组蛋白的示例参考文献(注：内容为假设性概括，仅供参考)：
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1. **标题**: *Recombinant Fibulin-7 promotes cell adhesion through integrin interaction*
**作者**: De Vega S. et al.
**摘要**: 研究通过重组表达Fibulin-7蛋白，证实其通过结合α5β1整合素调控细胞黏附，并参与细胞外基质的组装。
2. **标题**: *Fibulin-7 in dentinogenesis: Expression and functional analysis using recombinant protein*
**作者**: Yamada S. et al.
**摘要**: 利用重组Fibulin-7进行体外实验，揭示其在牙本质形成中促进成牙本质细胞分化和矿化的作用机制。
3. **标题**: *Recombinant Fibulin-7 suppresses tumor angiogenesis in a xenograft model*
**作者**: Huang L. et al.
**摘要**: 通过制备重组Fibulin-7蛋白，发现其抑制肿瘤血管生成的能力，可能成为抗肿瘤治疗的潜在靶点。
4. **标题**: *Structural and functional characterization of Fibulin-7 recombinant domains*
**作者**: Hirschfeld J. et al.
**摘要**: 解析重组Fibulin-7功能域的结构，阐明其与细胞外基质成分(如胶原IV)的结合特性及在组织修复中的作用。
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建议通过学术数据库(如PubMed、Google Scholar)以“Fibulin-7 recombinant”或“FBLN7 recombinant protein”为关键词检索真实文献。
FBLN7 (Fibulin-7) is a secreted extracellular matrix (ECM) glycoprotein belonging to the fibulin family, which plays critical roles in tissue development, homeostasis, and repair. Structurally, it contains calcium-binding epidermal growth factor (cbEGF)-like domains and a C-terminal fibulin-type module, enabling interactions with other ECM components such as laminins, proteoglycans, and integrins. These interactions facilitate cell adhesion, migration, and signaling, contributing to the assembly and stability of basement membranes and elastic fibers.
Fibulin-7 is prominently expressed during embryonic development, particularly in tissues undergoing morphogenesis, such as tooth buds, blood vessels, and neural crest-derived structures. Postnatally, its expression becomes more restricted but remains vital in maintaining tissue integrity. Dysregulation of FBLN7 has been implicated in pathological conditions, including vascular anomalies, periodontal disease, and tumor progression, where altered ECM dynamics promote invasion or metastasis.
Recombinant FBLN7 protein is engineered through molecular cloning, typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications (e.g., glycosylation). This allows researchers to study its biochemical properties, receptor binding, and functional roles in vitro and in vivo. Applications include investigating ECM remodeling mechanisms, screening for therapeutic targets in fibrosis or cancer, and developing biomaterials for regenerative medicine. Its ability to modulate cell-ECM crosstalk also positions it as a potential diagnostic or prognostic biomarker in diseases with ECM dysfunction. Overall, recombinant FBLN7 serves as a valuable tool for dissecting ECM biology and exploring translational interventions.
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