Cat: PA1000-8314

Recombinant Human SLC Protein,His

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关键信息

  • 基因名

    SLC

  • 应用

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    SLC;NBAT;Amino acid transporter heavy chain SLC3A1

  • 种属

    Human

  • 表达系统

    E. coli

  • 标签

    His tag N-Terminus

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    O00585

  • 表达区间

    24-134aa

  • 氨基酸序列

    SDGGAQDCCLKYSQRKIPAKVVRSYRKQEPSLGCSIPAILFLPRKRSQAELCADPKELWVQQLMQHLDKTPSPQKPAQGCRKDRGASKTGKKGKGSKGCKRTERSQTPKGP

  • 分子量

    43.8 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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背景信息

SLC (Solute Carrier) proteins are a diverse family of membrane transporters that play crucial roles in the uptake and export of a wide range of solutes, including ions, sugars, amino acids, and neurotransmitters. These proteins are essential for maintaining cellular homeostasis, regulating metabolism, and facilitating intercellular communication. Given their significant involvement in various physiological processes, including nutrient absorption, ion balance, and drug resistance, SLC proteins are of particular interest in both basic research and clinical applications. Recent studies have highlighted their potential implications in various diseases, such as metabolic disorders, cancer, and neurodegenerative conditions. Despite their importance, the functional characterization of SLC proteins has been hindered by challenges in recombinant expression, purification, and structural analysis. Consequently, research efforts have increasingly focused on developing robust methodologies for generating and studying SLC proteins in vitro. This includes optimizing expression systems, employing advanced purification techniques, and utilizing structural biology approaches to elucidate their mechanisms of action. By gaining a deeper understanding of SLC protein structure and function, researchers aim to uncover novel therapeutic targets and strategies for disease intervention, ultimately advancing the field of drug development and personalized medicine.

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